关键词: 杂交竹, 梢枯病, 毒素, 暗孢节菱孢, 生物活性

Abstract:

The toxin production conditions of Arthrinium phaeospermum were studied by acquiring the crude protein toxin with the methods of liquid shake culture and ammonium sulphate precipitation. According to impregnation method and disease index as the evaluation criteria, bioassay was tested in hybrid bamboo. The results showed that Fries culture medium was the optimal culture of toxin production, and the disease index reached 70.67%. Different culture time and temperature had greater effects on the amount of toxin production of this fungus, whose optimal time and temperature were 12d and 25℃. It had no special requirement for light, the satisfied dissolved oxygen content was between 120mL to 160mL, and the amount of toxin production was most at pH8.0. Under the optimized conditions, the ability of toxin production of this fungus had significantly increased, and the disease index of hybrid bamboo was up to 89.12%.

Key words: hybrid bamboo, blight, toxin, Arthrinium phaeospermum, biological activity