野生荞麦籽粒中芦丁降解酶的分离纯化

四川农业大学学报 ›› 2011, Vol. 29 ›› Issue (03): 391-391.

野生荞麦籽粒中芦丁降解酶的分离纯化

唐宇¹,邵继荣²,罗庆林¹,郑亚迪²,孙俊秀³

1.
2. 四川农业大学
3. 四川烹饪高等专科学校

收稿日期:2011-06-20 修回日期:2011-08-15 出版日期:2011-09-30 发布日期:2011-10-13
通讯作者: 邵继荣
基金资助:
荞麦野生种质资源的保护和利用研究;苦荞异黄酮代谢途径关键酶基因的克隆

Purification of rutin degrading enzyme from wild buckwheat kernels

Received:2011-06-20 Revised:2011-08-15 Online:2011-09-30 Published:2011-10-13
Contact: SHAO Jirong

摘要/Abstract

摘要：

从野生荞麦种金荞麦籽粒中提取出芦丁降解酶，具有将芦丁降解为槲皮素的作用。该粗酶液用葡聚糖凝胶柱G-100和DEAE阴离子交换柱分离纯化后，最终酶的比活性由59.2 U/mg提高到671.4U/mg，纯化倍数和回收率分别为11.3倍和3.5%。所得酶液经SDS-PAGE检测酶分子量大小为60kDa左右。对酶液反应条件进行研究，发现酶的最适温度为40℃，最适pH值为5.0。在工业生产上可借助芦丁降解酶以酶解法取代酸解法生产槲皮素。

关键词: 野生荞麦, 金荞麦, 芦丁降解酶, 槲皮素, 芦丁

Abstract:

The rutin degrading enzyme which was extracted from seeds of Fagopyrum cymosum, one of wild buckwheat species, could effectively degrade rutin into quercetin. The rutin-degarding enzyme was purified with Sephadex G-100 and DEAE cellulose-32 anion exchange column. The enzyme was purified to 11.3-fold with 3.5% recovery, and the specific activity increased from 59.2 U/mg to 671.4 U/mg. SDS-PAGE analysis revealed a single protein band corresponding to a molecular mass of 60 KDa. Maximum enzyme activity was obtained at 40℃ and pH 5.0. Based on the results in this study, the rutin-degrading enzyme has great potential to replace the classical acid-hydrolytic method to produce quercetin in industrial application.

Key words: wild buckwheat, Fagopyrum cymosum, rutin degrading enzyme, quercetin, rutin

唐宇邵继荣罗庆林郑亚迪孙俊秀. 野生荞麦籽粒中芦丁降解酶的分离纯化[J]. 四川农业大学学报, 2011, 29(03): 391-391.

[1]	唐宇, 邵继荣, 罗庆林, 郑亚迪, 孙俊秀. 野生荞麦籽粒中芦丁降解酶的分离纯化[J]. 四川农业大学学报, 2011, 29(03): 391-396.
[2]	赵海霞, 白悦辰, 陈惠, 吴琦. 5份金荞麦核糖体基因内转录间隔区(ITS)序列分析[J]. 四川农业大学学报, 2011, 29(03): 299-303.
[3]	曾荣, 张健, 谭向红, 罗学刚. 分光光度法测定尤曼桉中的芦丁含量[J]. 四川农业大学学报, 2005, 23(02): 186-188.