关键词: 金荞麦, ITS, PCR, 序列分析

Abstract:

The genetic relationship among different Fagopyrum cymosum was revealed by analyzing the internal transcribed spacer (ITS) sequences of five F. cymosum samples. The sequencing results showed that there were about 700 bp including the whole ITS1, 5.8S and ITS2 regions. The further analysis indicated that ITS regions of five samples were 638 bp~797 bp in length. With lack of gap as treatment, the whole ITS sequence consisted of 488 conserved sites, 248 variable sites, 67 information sites and 175 singleton sites. The sequence similarity among thirteen F. cymosum samples varies from 86.8% to 98.8%, and the main variation occurs on ITS1 area. The data meant there was the abundant genetic diversity among different sources of F. cymosum. Phylogenetic tree was constructed by neighbour-joining method, which enriched genetic diversity of buckwheat at the molecular level. Analysis Results showed that F. cymosum was mainly divided into two groups, and the phylogenetic distance has some correlation with the locality of collection.

Key words: F. cymosum, ITS sequences, PCR, Sequence Analysis