藏猪兰尼定受体（RYR1）基因表达的发育性变化

四川农业大学学报 ›› 2011, Vol. 29 ›› Issue (02): 249-252.

藏猪兰尼定受体（RYR1）基因表达的发育性变化

李芳琼¹,蒋岸岸²

1.
2. 四川农业大学

收稿日期:2010-07-20 修回日期:2011-04-07 出版日期:2011-06-30 发布日期:2011-06-30
通讯作者: 李芳琼
基金资助:
农业部行业科研专项项目;农业部现代农业产业技术体系建设专项

Developmental expression changes of RYR1 gene in longissimus dorsi muscle of the Tibetan pigs

Received:2010-07-20 Revised:2011-04-07 Online:2011-06-30 Published:2011-06-30

摘要/Abstract

摘要： 采用实时荧光定量PCR方法检测了藏猪背最长肌组织中RYR1基因mRNA表达量在2、4、6和8月龄间的差异。结果表明：不同月龄藏猪背最长肌中RYR1基因mRNA的表达量存在明显的差异，表现为波动起伏。其表达量在2月龄时最高，4月龄表达量较2月龄极显著下降（P<0.01），6月龄又极显著上升（P<0.01），至8月龄极显著降低到最低水平（P<0.01）。本文初步揭示了藏猪RYR1基因的发育表达模式，为进一步研究RYR1基因提供了基础数据。

关键词: 罗格列酮, 血清；脂肪形成；前脂肪细胞；基因表达；猪

Abstract: To investigate the developmental expression changes of RYR1 gene at the age of 2, 4, 6 and 8 months in longissimus dorsi muscle of the Tibetan pigs, real-time quantitative PCR was applied. The results indicated that the expression level of RYR1 gene was highest and lowest at the age of 2 and 8 months （P<0.01）, respectively, then declined significantly from the age of 2 to 4 months（P<0.01）, increased significantly from the age of 4 to 6 months（P<0.01）, but no significant expression changes was observed between the age of 2 and 6 months. These results highlight the expression patterns of RYR1 gene and provide some data on which to base further study of the molecular basis of skeletal muscles growth.

Key words: Rosiglitazone, Serum；Adipogenesis；Preadipocyte；mRNA expression；Swine

李芳琼蒋岸岸. 藏猪兰尼定受体（RYR1）基因表达的发育性变化[J]. 四川农业大学学报, 2011, 29(02): 249-252.