关键词: 丙烯酰胺, 高效液相色谱, 固相萃取, 淀粉类食品

Abstract: A simple and rapid method using ion-exchange high performance liquid chromatography (HPLC) coupled with diode array detection (DAD) was developed for the determination of acrylamide in starch-based foods. Acrylamide was extracted by 80% methanol, and cleaned by a Oasis HLB solid-phase extraction (SPE) cartridge. For analysis, a HC-75H⁺ cation ex change column was used with 0.005 mol/L sulfuric acid as mobile phase, flow rate was 0.6 mL/min and detection wavelength was set at 200 nm. The limit of detection was estimated at 30 μg/kg, and recoveries of acrylamide from samples spiked at levels of 100~1000 μg/kg ranged between 92% and 104%. Finally, a test for commercial products such as potato crisps, biscuits, Youtiao, Shaobing showed good precision of the method, with relative standard deviations below 9%, giving results similar with those reported in the literature.

Key words: acrylamide, HPLC, SPE, starch-based food