四川农业大学学报 ›› 2004, Vol. 22 ›› Issue (04): 386-388.doi: 10.16036/j.issn.1000-2650.2004.04.021

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辣椒材料亲缘关系的RAPD初步分析

程云, 李焕秀   

  1. 四川农业大学 林学园艺学院, 四川 雅安 625014
  • 收稿日期:2004-10-25 出版日期:2004-12-31 发布日期:2017-03-04

Simple RAPD Analysis of Genetic Relationship of Pepper

CHENG Yun, LI Huan-xiu   

  1. College of Forestry and Horticulture, Sichuan Agricultural University, Yaan 625014, Sichuan, China
  • Received:2004-10-25 Online:2004-12-31 Published:2017-03-04

摘要: 为明确16个辣椒种质资源的亲缘关系,利用RAPD技术对不同类型的辣椒种质资源亲缘关系进行了初步分析。从40个随机引物中筛选1个A2用于PCR反应,60%的扩增条带表现多态性,聚类结果表明,16份辣椒种质资源可以分为8大类,DNA分子水平上辣椒亲缘关系的分析和传统研究结论基本一致。

关键词: 辣椒, 亲缘关系, RAPD

Abstract: The relative relationship of different types of pepper germplasm resources is studied simply by RAPD technology. Polymorphics is reflected in 44.4% of amplified bands from 1 random primer selected from 40 employed in this experiment. 16 peppers germplasm resources can be classified into 8 groups by cluster analysis and the relationship by RAPD technology is similar to traditional methods.

Key words: pepper, genetic relationship, RAPD

中图分类号: 

  • S641.3