关键词: 籼稻, 成熟胚, 愈伤组织, 诱导, 状态调控

Abstract: Some factors which were involved in mature embryos callus culture in Indica Rice were studied in this experiment by using 7 varieties. The results indicate that both cold and hot pretreatment can greatly improve the frequency of callus induction. CC culture media is tested to be the best media for mature embryos callus induction. Different materials are different in the demands of 2, 4-D, but the concentration at 2 mg/L can commonly get satisfied results on induction. At the same time, another hormone ABA can be used to improve the condition of the callus. It is also found that the best replacing period of callus for almost all materials is about 12 days. Some additives, such as Pro, DMSO, Gln were tested can obviously decrease the browning rate of callus, but the effect of AgNO₃ is not investigated. At last, we can also maintain and improve the embryogenesis ability of callus by crossing culture media of NB and MS, by controlling the concentration of hormone 2, 4-D and ABA, and by creating circumstances through dry culture and permeating threaten treatment.

Key words: Indica Rice, mature embryo, callus, induction, condition regulation