关键词: 观赏植物, 组织培养, 玻璃化

Abstract: Significant progress in vitrification shoots of ornamental plant in the past decades is summarized in this paper. Characteristics of morphological, physiological and biochemical of vitreous shoots are presented. The major factors can result in vitrification in vitro as follows:explants; concentration of agar, sucrose, hormone, NH₄NO₃; sealing material; temperature; light; etc. The effective way to decrease the glass-like plantlet phenomenon is to choose proper explants and sealing material, to increase the concentration of sucrose and agar in medium, to decrease the concentration of hormone, and to form suitable environment conditions(temperature and light), and so on.

Key words: ornamental plant, tissue culture, vitrification