关键词: 麦类作物, C-带, 正交旋转组合设计

Abstract: The analyses were carried out by doubling orthogonal and rotate combination design for the five treated factors:0.2mol/L HCl, saturated Ba(OH)₂ solution, temperature of water to wash Ba(OH)₂ solution, and 2×SSC solution, which involved in the Giemsa-C banding procedure on the study Triticeae chromosomes. The statistical results indicate the most important factors to influence the banding pattern on the Triticeae chromosomes are the treatment of both the 0.2mol/L HCl and saturated Ba(OH)₂ solution in the Giemsa-C banding technique. The function of alcohol treatment might be increasing the crop chromosome tolerance of saturated Ba(OH)₂ treatment. Therefore, it is no necessity for the treatment of alcohol when a little treated time of saturated Ba(OH)₂ solution is reduced, but in order to obtain better results in the Triticeae chromosome Giemsa C-banding procedure, it appears to be the adopting steps that are the treatments of 0.2mol/L HCl with range of 2.33 to 2.48 minutes in 60℃, and saturated Ba(OH)₂ with 7.11 to 7.39 minutes in 18℃, and 2×SSC with 1.30 to 1.43 hour in 56~60℃. However, the alcohol treatment dodes not seem to be important in the C-banding technique while the treating time of Ba(OH)₂ is reduced a little. The water temperature to wash Ba(OH)₂ solution is better to control in 30~40℃ in order not to affect the final results.

Key words: TRITICEAE CROP, C-BANDING, ROTATION, ORTHOGONAL COMBINATION DESIGN