关键词: 鹅观草属, 披碱草属, 仲彬草属, 猬草属, RAPD分析, 亲缘关系, 基因组

Abstract: Fifteen species of Roegneria, Elymus, Hystrix and Kengyilia(Poaceae:Triticeae) were analyzed by using random amplified polymorphic DNA(RAPD) markers. 34 decamer oligonucleotides random primers from Opron Technologies were used for polymorphic selection. 27(79.4%) produced polymorphic products. A total of 213 bands amplified from 16 primers were selected for RAPD analysis. The data were used to generate Nei's similarity coefficients and to construct a dendrogram by using UPGMA in NTSYS programs. It is concluded as follows:①The species of Roegneria, Elymus, Hystrix and Kengyilia are clustered in four groups, which represent Roegneria, Elymus, Hystrix and Kengyilia respectively on 0.6 of genetic similarity coefficients; ②The relationships between the species of Hystrix and the species of Roegneria and Kengyilia are remote; ③Genetic diversity exists between Elymus and Hystrx although they possess similar StH genome; ④The species of StY and StYH genomes in Roegneria are clustered together, which suggests they have closer relationships; ⑤There is certain RAPD genetic diversity in the species of StY genomes of Roegneria; ⑥Kengyilia is related to Roegneria; ⑦RAPD results are basically comparable with those obtained from studies on morphology and cytology. The present study discusss the usefulness of RAPD markers in the systematic studies of Triticeae.

Key words: ROEGNERIA, ELYMUS, KENGYILIA, HYSTRIX, RAPD ANALYSIS, PHYLOGENETIC RELATIONSHIP, GENOME