关键词: 葡萄柚, 离体培养, 根, 不定芽

Abstract: [Objective] The objective of the study was to establish regeneration system of C. paradisi in vitro and to provide useful references for the application of genetic transformation technique. [Method] The roots of aseptic seedlings germinated from the seeds of 10 grapefruit varieties were cultured in vitro to obtain regeneration plants in the medium with different hormones and concentrations. [Results] The callus were observed in the medium MS+0.5 mg/L 6-BA+0.5 mg/L NAA and Perlist had the highest callus induction frequency (87.95%).The differentiation of adventitious buds was observed in the medium MS+2.0 mg/L 6-BA+0.5 mg/L NAA and Star Ruby had the largest differentiation rate of adventitious buds (37.84%).The regeneration buds were multiplied in the medium MS+2.0 mg/L 6-BA+0.2 mg/L NAA. The adventitious roots were induced in the medium 1/2 MS+1.0 mg/L IBA and the regeneration plants finally were obtained.The survival rates of regenerative plant ranged from 75.47% to 88.40%. [Conclusion] The regeneration systems of 6grapefruit varieties were established based on the study.

Key words: Citrus paradisi Macf., culture in vitro, root, adventitious bud