大花蕙兰组培苗耐寒性的综合评价

doi:10.3969/j.issn.1000-2650.2014.03.007

摘要/Abstract

摘要： 【目的】比较‘黄金岁月’(Cymbidium Lovely Moon 'Crescent')、‘红霞’(Cymbidium Royal Red 'Princess Nobuko')和‘晨辉’(Cymbidium Twilight Moon 'Daylight')3个品种大花蕙兰耐寒性的强弱,筛选大花蕙兰耐寒性鉴定的生理指标。【方法】采用人工模拟低温的方法,设置10、5、0和-5 ℃共4个低温处理,探讨其对3个品种大花蕙兰地上部和地下部丙二醛(MDA)、膜透性(CMP)、可溶性蛋白质(SP)、可溶性糖(SS)、超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)等7项生理指标的影响,利用主成分分析、隶属函数法和逐步回归分析,对这7个耐寒性生理指标进行综合评价。【结果】3个不同品种大花蕙兰在受到低温胁迫时,3种酶活性有不同程度升高,其中SOD和POD活性增加幅度最为明显,其耐寒性强弱的排序为:‘黄金岁月’＞‘红霞’＞‘晨辉’。【结论】从不同生理指标的变化趋势来看,细胞膜透性、SOD和POD活性的变化趋势可反映出不同品种的大花蕙兰组培苗的耐寒性的强弱,因此,可将细胞膜通透性、SOD和POD活性作为其耐寒性的鉴定指标。地上部和地下部在反映组培苗耐寒性能方面存在差异性。

关键词: 大花蕙兰, 耐寒性, 主成分分析, 综合评价

Abstract: 【Objective】 The aim of the study was to compare cold resistance among three varieties of C.hybridum and to select physiological indicators of cold-resistance of C.Hybridum. 【Method】 This study included four low temperature treatments (10, 5, 0 and -5 ℃) by artificial simulation method. The effects of low temperatures on malondialdehyde (MDA), Membrane permeability (CMP), soluble protein (SP), soluble sugar (SS), superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) activities of aboveground and underground parts of three C.hybridum varieties were investigated. The seven cold-resistance physiological indexes were comprehensively evaluated using principal component analysis, membership function analysis and stepwise regression analysis. 【Results】 Three C.hybridum when subjected to cold stress, there are three kinds of activity increased to varying degrees, of which the magnitude of SOD and POD activity increased the most obvious, the order of cold-resistance capacity of varieties was Lovely C. Moon 'Crescent' ＞ C.Royal Red 'Princess Nobuko' ＞ C.Twilight Moon 'Daylight'. 【Conclusion】 CMP, SOD and POD activities were significantly associated with cold resistance, and thought be the indexes to identify cold resistance in C.hybridum. There were differences in cold-resistance between aboveground and underground components.

Key words: Cymbidium hybridum, cold resistance, principal component analysis, comprehensive evaluation

中图分类号:

S688.2

唐敏,蒋天仪. 大花蕙兰组培苗耐寒性的综合评价[J]. 四川农业大学学报, 2014, 32(3): 283-287.

TANG Min, JIANG Tian-yi. Comprehensive Evaluation on Cold Resistance of Cymbidium hybridum Varieties[J]. jsau, 2014, 32(3): 283-287.

参考文献

[1] 文磊, 谭美, 王四清. 大花蕙兰营养生长期植株生长与辐热积关系的模拟模型研究[J]. 园艺学报, 2014, 41(5): 1001-1008.
[2] 刘园, 王四清. 大花蕙兰(Cymbidium hybridum)的研究动向[J]. 园艺学报, 2005, 32(4): 748-752.
[3] Blidar C F, Bandici G E, Szabo I, et al. In vitro reactivity of Cymbidium hybridum L.protocorms, on bistratified culture media, using various supernatant sucroses solution [J]. Analele Stiintificeale Universitatii Ovidius Constanta: Seria Matematica, 2009, ⅩⅥ(1): 31-37.
[4] 易绮斐, 邢福武, 叶秀粦. 大花蕙兰‘黄金小神童’花期研究初探[J]. 园艺学报, 2005, 32(3): 531-533.
[5] 赵九洲, 宛成刚, 陈洁敏, 等. 不同基质的水分亏缺对大花蕙兰生理生化特性的影响[J]. 南京林业大学学报(自然科学版), 2003, 27(2): 29-32.
[6] 金丽丽, 孙龙生. 花卉抗寒性研究方法和测定指标概述[J]. 辽宁农业科学, 2005(6): 37-39.
[7] 杨凤翔, 金芳, 颜霞. 不同草莓品种抗寒性综合评价[J]. 果树学报, 2010, 27(3): 368-372.
[8] 张宪政. 作物生理研究法[M]. 北京: 农业出版社, 1992: 144-155.
[9] 郝建军, 康宗利, 于洋. 植物生理学实验技术[M]. 北京: 化学工业出版社, 2007: 141-142.
[10] 李合生. 植物生理生化实验原理和技术[M]. 北京: 高等教育出版社, 2003: 164-263.
[11] 李丰先, 周宇飞, 王艺陶, 等. 高粱品种萌发期耐碱性筛选与综合鉴定[J]. 中国农业科学, 2013, 46(9): 1762-1771.
[12] 王贺正, 马均, 李旭毅, 等. 水稻开花期抗旱性鉴定指标的筛选[J]. 作物学报, 2005, 31(11): 1485-1489.
[13] 柳新红, 何小勇, 苏冬梅, 等. 翅荚木种源抗寒性综合评价体系的构建与应用[J]. 林业科学, 2007, 43(10): 45-50.
[14] 吕优伟, 贺佳圆, 白小明, 等. 9个野生早熟禾对低温胁迫的生理响应及苗期抗寒性评价[J]. 草地学报, 2014, 22(2): 326-333.
[15] 周广生, 梅方竹, 周竹青, 等.小麦不同品种耐湿性生理指标综合评价及其预测[J]. 中国农业科学, 2003, 36(11): 1378-1382.
[16] 张朝阳, 许桂芳. 利用隶属函数法对4种地被植物的耐热性综合评价[J]. 草业科学, 2009, 26(2): 57-60.
[17] 吕跃东, 董凤祥, 王贵禧, 等. 平欧杂交榛抗寒性综合评价体系的建立与应用[J]. 林业科学, 2008, 44(9): 31-35.
[18] Patton A J, Cunningham S M, Volenec J J, et al. Differences in freeze tolerance of zoysiagrasses:Ⅱ.Carbohydrate and proline accumulation [J]. Crop Science, 2007, 47(5): 2170-2181.
[19] Ederli L, Reale L, Ferranti F, et al. Responses induced by high concentration of cadmium in Phragmites australis roots [J]. Physiologia Plantarum, 2004, 121: 66-74.
[20] Dionne J, Castonguay Y, Nadeau P, Desjardins Y. Freezing tolerance and carbohydrate changes during cold acclimation of green-type annual bluegrass (Poa annua L.) ecotypes [J]. Crop Science, 2001, 41(2): 443-451.
[21] 相昆, 张美勇, 徐颖, 等. 不同核桃品种耐寒特性综合评价[J]. 应用生态学报, 2011, 22(9): 2325-2330.
[22] 何西凤, 杨途熙, 魏安智, 等. 自然越冬过程中花椒抗寒性生理指标的变化[J]. 东北林业大学学报, 2009, 37(5): 67-71.