关键词: 金银花, 指纹图谱, 绿原酸, 木犀草苷

Abstract: [Objective] This study established HPLC chromatographic fingerprint of Lonicerae japonica and measured the contents of chlorogenic acid and galuteolin from different areas.The objective was to comprehensively evaluate L. japonica quality. [Method] The Aglient TC-C₁₈ column (4.6 mm×250 mm,5 μm) and gradient elution was used in this study.The mobile phase was composed of 1% HAC (A) and acetonitrile (B).The elution processes were shown as followed:0-5 mins,A 95%→92%;5-7 mins,A 92%→87%;7-28 mins,A 87→85%;28-38 mins,A 85→84%;38-43 mins,A 84→82%;43-53 mins,A 82→72%;53-63 mins,A 72→50%;63-65 mins,A 50→5%;75 min,A 5%.The flow rate was 0.8 mL/min.The detection wavelength was 250 nm and column temperature of 30℃.Data analysis was performed using professional software named Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine composed by Chinese Pharmacopoeia Committee (Version 2004A). [Results] Fifteen common peaks were selected as fingerprints of L. japonica there were good similarities (>0.9)between standard fingerprint and the fingerprint from 10 medicinal materials.Underthe established conditions,the chlorogenic acid and galuteolin were separated well.Additionally,all calibration curves showed good linear regression and their recovery were 99.2% and 99.4%,respectively. [Conclusion] The method is sensitive,accurate and convenient and it can be used to evaluate L. japonica quality.

Key words: Lonicerae japonica, fingerprint, chlorogenic acid, galuteolin