关键词: 猪精子, 冷冻保护剂, Caspases表达模式

Abstract: [Objective] To explore Caspases expression prolifes in boar spermatozoa during cryopreservation with different cryoprotectants (CPAs). [Method] This study firstly investigated all 15 Caspases expressions on boar spermatozoa cryopreservation with different CPAs by RT-PCR and qRT-PCR. [Results] ① Glycerol and ethylene glycol can inhibit the expression of Caspase-2,-8, and-13,while DMSO can only inhibit the expression of Caspase-2,-8.② Trehalose and lactose can effectively inhibit the expression of Caspase-2,-8,-13 and-15,while sucrose can effectively inhibit the expression of Caspase-1,-6,-13 and-15.③ SOD and catalase can effectively prevent Caspase-2,-8,and-13from expression.But glutathione was proved effectively in inhibiting the expression of Caspase-13 alone.④ Neither Caspase-3or Caspase-7 was responsible for any CPAs. [Conclusion] The influences of cryopreservation on boar spermatozoa were mainly focus on expression of initiator Caspases.Supplement of glycerol,sucrose and lactose were still reasonable to prevent the boar spermatozoa from apoptosis.

Key words: boar spermatozoa, cryopreservation, Caspases expression pattern