关键词: 绿色粘帚霉, 厚垣孢子, 柑橘绿霉

Abstract: [Objective] The Gliocladium virens strain UV-44was used as the object of study.Our aim was to investigate the effects of different culture mediums,pH,incubation time and temperature on chlamydospore production and it's control effects on Penicillium digitatum.[Method] The strain was cultivated by shaking flask and the number of chlamydospore was calculated by hemocytometer.The oranges were individually wrapped after dipping in solutions.The relative control effects of chlamydospore suspension,conidia suspension and fermentation liquid on P. digitatum were measured compared to controls (water and Prochloraz).[Results] Under the conditions (oatmeal,pH 10,13dand 30℃),the concentration of UV-44chlamydospore was 1.52×10¹¹ A/L,which value was the largest.The relative control effect increased with time after 30d,60d and 90d.However,the concentration of chlamydospore in conidial suspension and fermentation liquid were decreased gradually.[Conclusion] Chlamydospore of G. virensis valuable for biocontrol.

Key words: Gliocladium virens, chlamydospore, Penicillium digitatum