四川农业大学学报 ›› 2013, Vol. 31 ›› Issue (04): 393-397.doi: 10.3969/j.issn.1000-2650.2013.04.006

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油樟油主成分对几种常见病原菌的抑菌活性研究

张萍1, 王平2, 石超峰2,3, 魏琴2, 杜永华2, 周黎军2, 殷中琼3, 贾仁勇3   

  1. 1. 宜宾学院生命科学与食品工程学院, 西南特色经济植物保护与利用重点实验室, 四川 宜宾 644000;
    2. 四川省简阳中学, 四川 简阳 641400;
    3. 四川农业大学动物医学院, 四川 雅安 625014
  • 收稿日期:2013-08-21 出版日期:2013-12-31 发布日期:2017-02-28
  • 作者简介:张萍,硕士。
  • 基金资助:
    四川省科技厅支撑项目(2009FZ0090);四川省教育厅成果转化培育项目(11ZZ022)

Antibacterial Activity of Essential Components of Cinnamomum longepaniculatum Oil on Several Typical Pathogenic Bacteria

ZHANG Ping1, WANG Ping2, SHI Chao-feng2,3, WEI Qin2, DU Yong-hua2, ZHOU Li-jun2, YIN Zhong-qiong3, JIA Ren-yong3   

  1. 1. Department of Life Science and Food Engineering, Yibin University., Key Lab of Southwest Special Economic Plant Protection Utilization, Yibin 644000, Sichuan, China;
    2. Jianyang School, Jianyang 641400, Sichuan, China;
    3. College of Veterinary Medicine, Sichuan Agricultural University, Yaan 625014, Sichuan, China
  • Received:2013-08-21 Online:2013-12-31 Published:2017-02-28
  • Contact: 魏琴,博士研究生,教授,研究方向:资源植物学,E-mail:weiqin2001-67@163.com。 E-mail:weiqin2001-67@163.com

摘要: [目的] 为了测定油樟油中1,8-桉叶油素(99.5%)、松油烯-4-醇(99%)和γ-松油烯(99%)主成分的抑菌效果。[方法] 采用二倍释法测定其对几种常见病原菌沙门氏菌、大肠杆菌和金黄色葡萄球菌的最低抑菌浓度(minimal inhibitory concentration,MIC)和最低杀菌浓度(minimal bactericidal concentration,MBC),利用沙氏琼脂平板稀释培养法绘制其抑菌曲线。[结果] 发现松油烯-4-醇对3种试验菌的抑菌效果最明显,其次是1,8-桉叶油素,γ-松油烯仅对沙门氏菌有较好的抑菌活性,对大肠杆菌和金黄色葡萄球菌抗菌活性较差。[结论] 3种油樟油单体的杀菌效果均呈现时间-浓度依赖型。

关键词: 油樟, 1, 8-桉叶油素, 松油烯-4-醇, γ-松油烯, 抑菌

Abstract: [Objective] The aim of the study was to measure the antibacterial activity of essential components of Cinnamomum longepaniculatum oils,such as 1,8-cineole (99.5%),terpinene-4-alcohol (99%) and γ-terpinene (99%).[Method] Several typical pathogenic bacteria such as Salmonella enteritidis (Salmonella),Escherichia coli and Staphylococcus aureus (S. aureus) were used to determine the minimal inhibitory concentration and minimal bactericidal concentration,the growth curves were drawn using CFU dilution-plate method.[Results] The terpinene-4-alcohol exhibited the highest antibacterial activity to tested bacteria,followed by 1,8-cineole.γ-terpinene was effective to Salmonella,but ineffectual to E.coli and S. aureus.[Conclusion] The antibacterial activity of 3components of C. longepaniculatumoil all were dependent on time and concentration.

Key words: Cinnamomum longepaniculatum, 1,8-cineole, terpinene-4-alcohol, γ-terpinene, bac-teriostat

中图分类号: 

  • Q946.887