关键词: 巨大芽孢杆菌, 中性纤维素酶, 发酵条件, 响应面分析法

Abstract: [Objective] To solve the low yield,high production cost issues in fermentation industry,and speed up the pace of industrial application of cellulase.[Method] The research used response surface methodology to optimize the fermentation conditions of neutral cellulase production by aBacillus Megateriumgenetic engineering bacteria WH 320-pHIS1525-G7 stain in 5 L fermenter.[Results] The optimal fermentation conditions were 17.62% inoculation amount,37℃ fermentation temperature,pH 7.0,tank pressure 0.03-0.05 Mpa,rotation speed of shaker 300r/min,fed 11.33 hxylose by 0.41g/(L·h) flow rate,dissolved oxygen ≥ 30% during the fermentation process.[Conclusion] The cellulase activity was up to 2.152 U/mL which was 2times higher than the shaking culture.This research can provide the basis for the cellulase manufacture.

Key words: Bacillus Megaterium, neutral cellulase, fermentation conditions, response surface methodology