四川农业大学学报 ›› 2013, Vol. 31 ›› Issue (02): 204-207.doi: 10.3969/j.issn.1000-2650.2013.02.017

• • 上一篇    下一篇

喷雾干燥对人溶菌酶转基因山羊乳汁的影响

吴敏1, 刘思国2, 兰翀2, 徐旭俊2, 陈建泉2, 马恒东1, 成国祥2   

  1. 1. 四川农业大学动物科技学院, 四川 雅安 625014;
    2. 上海转基因动物育种与制药工程技术研究中心/上海杰隆生物工程股份有限公司, 上海 201210
  • 收稿日期:2013-03-12 出版日期:2013-06-30 发布日期:2017-02-28
  • 通讯作者: 刘思国,副研究员,主要从事转基因动物制药研究,E-mail:lsg@cngenon.com;马恒东,教授,主要研究方向:动物生理学,E-mail:mahengdong007@163.com。 E-mail:lsg@cngenon.com;mahengdong007@163.com
  • 作者简介:吴敏,硕士研究生。
  • 基金资助:
    上海市科学技术带头人项目(No.11XD1422000);上海市"科技创新行动计划"(No.11431921400)

Influence of Spray-drying on Human Lysozyme Transgenic Goat Milk

WU Min1, LIU Si-guo2, LAN Chong2, XU Xu-jun2, CHEN Jian-quan2, MA Heng-dong1, CHENG Guo-xiang2   

  1. 1. College of Animal Science and Technology, Sichuan Agricultural University, Yaan 625014, Sichuan, China;
    2. Shanghai Engineering Research Center for Transgenic Animal breeding and Pharmaceutical/Shanghai Genon Bioengineering Co. Ltd., Shanghai 201203, China
  • Received:2013-03-12 Online:2013-06-30 Published:2017-02-28

摘要: [目的] 揭示奶粉制备过程对转基因山羊乳汁中表达的重组人溶菌酶(recombinant human lysozyme,rhLYZ)的影响。[方法] 对表达有2mg/mL rhLYZ的转基因山羊乳汁进行了喷雾干燥,对获得的奶粉样品进行了溶解性,乳汁成分,蛋白组分以及溶菌酶活性的测定,对测定结果进行统计分析。[结果] 喷雾干燥引发非脂固形物的损失率为42.05%;整个热处理过程引发可溶性蛋白损失率为25.3%;乳汁中rhLYZ的损失为51.1%,扣除总干物质的损耗,喷雾干燥过程引发了约17.7%的rhLYZ生物学活性损失。[结论] 制备奶粉过程对转基因山羊乳汁中表达的人溶菌酶影响较小,喷雾干燥是人溶菌酶羊奶的一种可行的保存和加工方式。

关键词: 喷雾干燥, 人溶菌酶, 转基因山羊

Abstract: [Objective] The influence of milk powder preparation process on the physicochemical properties of transgenic goat milk and the recombinant human lysozyme (rhLYZ) were studied.[Method] The transgenic goat milk with 2 mg/mL of rhLYZ was treated by spray-drying.The solubility,milk composition,protein fractions,and lysozyme activity of milk samples were tested.The results were statistical analyzed.[Results] The lose rate of non-fat solids caused by spray-drying was up to 42.05%;the lose rate of dissolvable proteins was about 25.3%;and about 17.7% of rhLYZ biological activity was losted which resulted from the drying process.[Conclusion] The influence of milk powder preparation process on rhLYZ was mild,and the milk powder could be a feasible method for preserving and processing rhLYZ milk.

Key words: spray-drying, human lysozyme, transgenic goat

中图分类号: 

  • S879.1