关键词: 醇碱法, 响应面分析, 厚朴酚, 和厚朴酚

Abstract: [Objective] Optimize the extraction condition of highest yields of total phenols (magnolol,honokiol) for cortex Magnolia of ficinalis var. biloba,using the response surface analysis.[Method] The extraction factors of the concentration of ethanol,concentration of alkali,solid-to-liquid and periods of ultrasound-assisted extraction were employed.[Results] The second-order quadratic regression equation fitted the experimental data well with regard to magnolol (R²=0.940 2),honokiol (R²=0.962 6) and total phenols (R²=0.960 6),respectively.Under the condition of ultrasonic power 150 W,the optimum extraction conditions for total phenols were ethanol 56%,alkali 0.8%,solid-to-liquid 1:36,period of extraction 29 min,where herein yields of magnolol were 1.95 mg/g,honokiol were 9.80 mg/g,total phenols were 11.75 mg/g.[Conclusion] The predicted values got consistent with experimental values.Ethanol-alkali extraction of total phenols dominated in time,power and efficiency,comparing with ethanol and alkali extraction.

Key words: ethanol-alkali extraction, response surface analysis, magnolol, honokiol