关键词: 牛蛙, 腐皮病, 红腿病, 组织病理学

Abstract: [Objective] To observe the pathological changes of sick Rana catesbeiana.[Method] The pathological changes in Rana catesbeiana with natural rotten-skin and red-leg disease were observed by light microscope after using paraffin section and H·E staining.[Results] Gross lesions included some parts of the skin mostly on head and back were rotten;erythema was seen on abdomen and leg skin;the liver,spleen and kidney were swollen with severe hemorrhage;gastrointestinal tract was inflamed with yellowish mucus.The main histopathological characterization of sick frog was parenchymal cell necrosis,hemorrhage,inflammatory cell infiltration were existed in main organ,including skin and muscle,liver,spleen,kidney and gastrointestinal.Catarrhal-hemorrhagic inflammation was observed in gastrointestinal tract.The epithelial cells of stomach were necrosis and falling off.The hepatocytes swelled with severely vacuolar degeneration,focal necrosis with inflammatory cell infiltration appeared.The lymphocyte and reticuloendothelial cell were severe necrosis.Degeneration and necrosis were appeared in renal tubular epithelia.The renal interstitium and renal glomerulus were hyperemia severely.[Conclusion] The typical symptom of the disease included rotten skin of head and back,haemorrhage of belly and leg skin,inflammatory lesion of hepar,spleen,kindey,and gastrointestinal tract.The serious lesion of several organs is the cause of death of sick frog.

Key words: Rana catesbeiana, rotten-skin disease, red-leg disease, histopathology