关键词: 纤维素酶, 葛渣, 异黄酮提取

Abstract: [Objective] The objective of this study was to optimize the extraction conditions with cellulase pretreatment of isoflavones from pueraria root residue.[Method] Single-factor test and response surface methodology (RSM) were used to analysis the effects of the enzyme dosage,enzymolysis temperature,pH value and enzymolysis time on the extraction yield of isoflavones.[Results] The optimal enzymolysis conditions were got through the regression model,which are cellulase dosage 11 mg per 5 g pueraria root residue,enzymolysis temperature 51℃,pH 5.0,enzymolysis time 2.3 h.Under these conditions,the extraction yield of isoflavones can reach at 12.34 mg/g,which is increased 57% than that of traditional method.[Conclusion] The result shows that the optimal conditions with cellulase pretreatment can effectively improve the extraction amount of isoflavones from pueraria root residue.

Key words: cellulase, pueraria root residue, isoflavones extraction