关键词: 中草药, 免疫力, 鲫鱼, 腹注

Abstract: [Objective] Our study aimed at studying effects of Chinese herbal medicine on immunity of Crucian. [Method] More than six Chinese herbal medicines(ephedra, kuh-seng, astragalus mongholicus. etc) were grinded evenly then made into compound preparation by being boiled into medicinal juice after which the juices divided into 0(control group), 0.5%(A group), 2.0%(B group), 4.0%(C group) by percentage of mass with normal saline and then injected into crucian carps from intraperitoneal cavity. The blood collected on the 7^th, the 14^th, the 21^st, the 28^th day during the experiments for examining their immune indexs. [Results] the serum lysozyme activity in experimental groups was significantly higher than control group(P<0.05) since the first week, in addition, the higher the concentration was, its activity was higher accordingly;the serum bactericidal activity in experimental groups was significantly higher than control group(P<0.05), especially significant in group C, and the other two groups were at the same level;On the 7^th day, there was no significant difference between the number of NBT positive cells in group A and control group, but significant in group B and group C between control group;and since the 7^th day, all the experimental groups were significantly higher than control group, significance lied between all of them;On the 28^th day, difference turned insignificant. After the injection of herbal medicine, the serum cortisol level among crucian carps made some difference, the mass index of spleen was relatively increased, but the effects were not significant. [Conclusion] The Chinese herbal medicine can improve the crucian carp's non-specific immunity effectiveness.

Key words: Chinese herbal medicine, immunity, Carassius auratus, intraperitoneal injection