关键词: 淡紫拟青霉, 液体发酵, 条件筛选

Abstract: [Objective] Our aim was to optimize the culture conditions of Paecilomyces lilacinus liquid of enmatophagous fungus, and to obtain the optimal conditions of liquid fermentation of P. lilacinus. [Method] P. lilacinus liquid was cultured under different carbon sources, nitrogen sources, inorganic salts, pH values and times. The amount of spores in each treatment was measured. Optimal condition and time were selected. [Results] ① The optimal basic medium A1(g/L):glucose 40, NaNO₃ 20, KH₂PO₄ 0.5, MgSO₄·7H₂O 0.5;② The optimal compound medium b(g/L):glucose 40, NaNO₃ 10, (NH₄)₂SO₄ 5, soybean powder 5, KH₂PO₄ 0.5, MgSO₄·7H₂O 0.5;③ The best initial pH value was 6.0~7.0 and temperature was 26℃;④ The optimum time for fermentation was 72~80 h. [Conclusion] This study optimizes the culture conditions of P. lilacinus and provides foundation for large scale production of P. lilacinus.

Key words: Paecilomyces lilacinus, liquid fermentation, screening of fermentation conditions