关键词: 华山新麦草, AFLP标记, 遗传变异

Abstract: [Objective] The purpose of this study was to analyze the genetic variation of PHW-SA. [Method] 195 pairs of AFLP primers were used to amplify PHW-SA and its parents J-11(common wheat) and Psathyrostachys huashanica. [Results] 5 136, 3 953 and 5 637 markers were amplified in J-11, Psathyrostachys huashanica and PHW-SA, respectively, accounted for 29.0%, 22.3% and 31.8% of the total(17 726 banding), respectively;81.8% markers of J-11 and 49.6% markers of Psathyrostachys huashanica were amplified in PHW-SA;there were 2 484 co-markers between PHW-SA and J-11, which accounted for 48.4% and 44.1% of themselves, respectively;similarly, 246 co-markers existed between(6.2%) PHW-SA and(4.4%) Psathyrostachys huashanica;232 new markers which did not exist in its parents were amplified in PHW-SA and 66 markers of its parents could not be amplified in PHW-SA;there were 1 715 co-markers in all 3 materials, accounted for 33.4%, 43.4% and 30.4% of its total markers of J-11, Psathyrostachys huashanica and PHW-SA, respectively. [Conclusion] All evidence suggest that great genetic variation was occurred in the genome of PHW-SA when synthesized, and provide the theoretical information for wheat breeding in future.

Key words: Psathyrostachys huashanica, AFLP marker, hereditary variation