四川农业大学学报 ›› 2012, Vol. 30 ›› Issue (01): 92-97.doi: 10.3969/j.issn.1000-2650.2012.01.018

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绿原酸对建鲤生长、非特异性免疫功能和抗氧化能力的影响

张纯, 温安祥   

  1. 四川农业大学生命科学与理学院, 四川 雅安 625014
  • 收稿日期:2011-04-08 出版日期:2012-03-31 发布日期:2017-02-28
  • 作者简介:张纯,硕士研究生。
  • 基金资助:
    四川省教育厅重点项目(11ZA292)

Different of Chlorogenic Acid doses on Growth Performance, Non-specific Immunity and Antioxidant Functions of Cyprinus carpio var. Jian

ZHANG Chun, WEN An-xiang   

  1. College of Biology and Science, Sichuan Agricultural University, Yaan 625014, Sichuan, China
  • Received:2011-04-08 Online:2012-03-31 Published:2017-02-28
  • Contact: 温安祥,教授,硕士生导师,主要从事特种水生动物营养研究,E-mail:waxwbw@yahoo.com.cn。 E-mail:waxwbw@yahoo.com.cn

摘要: 试验研究绿原酸对建鲤生长、非特异性免疫及抗氧化能力的影响。选择均重为(62.93±5.82)g的建鲤360尾,随机分为5组(A~E组)。分别添加0、100、200、400和800mg/kg的绿原酸,于试验第10、20、30和40天取样,分别测定各组增重率、头肾指数、脾脏指数、白细胞吞噬率、血浆溶菌酶、超氧化物歧化酶、过氧化氢酶活性、总抗氧化能力及丙二醛含量。结果表明:第40天C组的增重率显著高于A组(P<0.05);各时段C组的头肾指数显著高于A组(P<0.05);第10、20和30天D组的脾脏指数显著高于A组(P<0.05);第10天,第20天和第30天C、D组的白细胞吞噬率显著高于A组(P<0.05);各时段D组血浆溶菌酶的活力显著高于A组(P<0.05);除第40天E组与A组的SOD差异不明显外(P>0.05),其余各组各时段的SOD均显著高于A组(P<0.05);第30、40天D组的CAT显著高于A组(P<0.05);第20、30和40天D组的T-AOC显著高于A组(P<0.05);第20、30和40天C、D组的MDA显著低于A组(P<0.05)。综合各指标的变化规律可知:①添加200mg/kg绿原酸能显著促进建鲤的生长;②添加200~400mg/kg绿原酸能显著增强建鲤的非特异性免疫和抗氧化能力;③长时间、高剂量添加绿原酸对建鲤的生长、非特异性免疫和抗氧化能力均有一定的抑制作用。

关键词: 绿原酸, 建鲤, 生长, 非特异性免疫功能, 抗氧化能力

Abstract: The experiment was aimed to investigate the effects of chlorogenic acid on growth performance, non-specific immunity and antioxidant functions of Cyprinus carpio var. Jian. 360 Cyprinus carpio var. Jian with an average weight of(62.93±5.82) g were selected, and were randomly divided into 5 groups(group A~E). 0, 100, 200, 400 and 800 mg/kg of chlorogenic acid were added into the diets of 5 groups, respectively. On the 10th, 20th, 30th, 40th day of the experiment, each group were sampled to measure weight gain rate, head kidney index, spleen index, phagocytic index of leukocyte, vitality of lysozyme, the SOD, CAT, T-AOC and MDA in blood. Results showed that: The 40th day, the weight gain rate of C group was significantly higher than that of A group(P<0.05);the head kidney index of C group was significantly higher than that of A group(P<0.05) at each time;the 10th, 20th and 30th day, the spleen index of D group was significantly higher than that of A group(P<0.05);the 10th, 20th and 30th day, the phagocytic rate of leukocyte of C group and D group was significantly higher than that of A group(P<0.05);each time the activity of plasma lysozyme of D group was significantly higher than that of A group(P<0.05);In addition, the SOD difference of group E and A was not apparent(P<0.05) on the 40th day, while the SOD of other groups in each period were significantly higher than that of A group(P<0.05). The 30th and 40th day, the CAT of group D was significantly higher than that of A group(P<0.05);the 20th, 30th and 40th day, the T-AOC of group D was significantly higher than that of A group(P<0.05);the 20th, 30th and 40th day, the MDA of C group and D group was significantly lower than that of A group(P<0.05). Comprehensive changes of the indicators showed that: ① Adding 200 mg/kg chlorogenic acid can significantly promote the growth of Cyprinus carpio var. Jian;② Adding 200~400 mg/kg chlorogenic acid can significantly enhance the nonspecific immune and antioxidant of Cyprinus carpio var. Jian;③ It has a certain degree of inhibition to the growth of Cyprinus carpio var. Jian and non-specific immunity and antioxidant function to add a high dose of chlorogenic acid for a long period of time.

Key words: chlorogenic acid, Cyprinus carpio var.Jian, growth, non-specific immunity function, antioxidant function

中图分类号: 

  • S963