关键词: 杂交竹, 梢枯病, 毒素, 暗孢节菱孢, 生物活性

Abstract: The toxin production conditions of Arthrinium phaeospermum were studied by acquiring the crude protein toxin with the methods of liquid shake culture and ammonium sulphate precipitation.Using impregnation method and disease index as the evaluation criteria, bioassay was tested in hybrid bamboo.The results showed that fries culture medium was the optimal culture of toxin production, with the disease index amounting to 70.67%.Different culture time and temperature had great effects on the amount of toxin production of this fungus, whose optimal time and temperature were 12 d and 25℃.It had no special requirement for light, the satisfactory dissolved oxygen content was between 120 mL to 160 mL, and the amount of toxin production was most at pH 8.0.Under the optimized conditions, the ability of this fungus to produce toxin had been increased significantly, and the disease index of hybrid bamboo was up to 89.12%.

Key words: hybrid bamboo, blight, toxin, Arthrinium phaeospermum, biological activity