关键词: 藏猪, 背最长肌, 兰尼定受体基因, 实时荧光定量PCR

Abstract: Rynodine receptor(rynodine receptor 1, RYR1) gene, also known as the halothane gene, which has been proved the major gene induced the porcine stress syndrome.The expression of the gene were associated with the pork quality traits deterioration, and thus has a more important research prospects.To investigate the regular changes of the gene expression for individuals in different developmental stages and further study the relationship between the gene and the meat quality traits, the real-time quantitative PCR was applied to test the developmental expression changes of RYR1 gene at the age of 2, 4, 6 and 8 months in Longissimus dorsal muscle of the Tibetan pigs.The results indicated that the expression level of RYR1 gene had been fluctuating at different ages, and which was highest expression at the age of 2 months, but decreased significantly(P<0.01) at 4 months compared with 2 month old, however, highly significant increased at 6 months old(P<0.01), and reduced significantly(P<0.01) to the lowest level at 8 months.These results initial revealed the expression patterns of RYR1 gene and provided some basic data for further study RYR1 gene.

Key words: Tibetan pigs, Longissimus dorsi muscle, rynodine receptor 1 gene, quantitative real-time PCR