四川农业大学学报 ›› 2011, Vol. 29 ›› Issue (01): 103-107.doi: 10.3969/j.issn.1000-2650.2011.01.020

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人工养殖大鲵病原荧光假单胞菌的分离鉴定

杜宗君1, 黄小丽1, 邓永强2, 汪开毓3, 耿毅3, 陈德芳1   

  1. 1. 四川农业大学动物科技学院水产系, 四川 雅安 625014;
    2. 四川省动物疫病预防控制中心, 成都 614000;
    3. 四川农业大学鱼病研究中心, 四川 雅安 625014
  • 收稿日期:2011-03-06 出版日期:2011-03-31 发布日期:2017-04-11
  • 基金资助:
    中央级农业公益性行业科研专项经费项目(200803013);教育部"长江学者和创新团队发展计划"创新团队项目(IRT0848)

Isolation and Identification of Pseudomonas fluorescents from Andrias davidianus

DU Zong-jun1, HUANG Xiao-li1, DENG Yong-qiang2, WANG Kai-yu3, GENG Yi3, CHEN De-fang1   

  1. 1. Department of Aquaculture, College of Animal Science and Technology, Sichuan Agricultural University, Yaan 625014 Sichuan, China;
    2. Sichuan Provincial center for Animal Disease Prevention and Control, Chengdu 610041, China;
    3. College of Veterinary Medicine, Sichuan Agricultural University, Yaan 625014, Sichuan, China
  • Received:2011-03-06 Online:2011-03-31 Published:2017-04-11

摘要: 从四川洪雅人工养殖的患病大鲵(Andrias davidianus)肝、肾中分离到一株优势菌(S100814),以腹腔注射、浸泡的方式进行人工感染试验,证实其为养殖大鲵的病原菌。该分离株生理生化特性与荧光假单胞菌一致,该菌为革兰氏阴性短杆菌,在TSA平板上生长呈边缘整齐、半透明的圆形灰白色菌落。接触酶、精氨酸双水解酶、明胶液化等为阳性,吲哚、V-P反应为阴性,可分解葡萄糖、阿拉伯糖、山梨醇、肌醇,但不发酵甘露糖、乳糖、甘露醇、麦芽糖,不产H2S。根据分离株的形态、生理生化特性,结合16SrDNA序列(GenBank登录号为HQ880245)与系统发育树分析,将其鉴定为荧光假单胞菌。药敏试验表明,菌株对强力霉素和新生霉素高度敏感,对恩诺沙星、庆大霉素、丁胺卡那和氟苯尼考耐药。

关键词: 大鲵, 荧光假单胞菌, 分离, 鉴定

Abstract: Bacterial strain S100814 was isolated from cultured Andrias davidianus in Hongya Sichuan Province.The strain was proved to be the pathogen of the disease by artificial infection test.The isolated strain was identified as Pseudomonas fluorescents by morphology, physiology, as well as 16SrDNA sequence(Accessible Number in GenBank:HQ880245).The isolated strain in TSA was hoar circular translucent colonies.The stain was short gram-negative bacterium.Glucose, arabinose, sorbitol, inositol, contact enzyme, two-arginine hydrolase, liquefied gelatin were positive;Maltose, lactose, mannitol, indole reaction, V-P test, maltose, H2S production were negative.It's physiological and biochemical characteristics were same with Pseudomonas fluorescence.The results showed that the strain was sensitive to doxycycline and novobiocin and resistance to enrofloxacin, gentamycin, amikacin, and florfenicol.

Key words: Andrias davidianus, Pseudomonas fluorescents, isolation, identification

中图分类号: 

  • S947.3