关键词: 马铃薯, 愈伤组织, 不定芽, 激素组合, 再生体系

Abstract: The regeneration system of six potato varieties was established with leaves as explants.The results showed that 6-BA and GA₃ both had notably effect on callus growth and adventitious bud differentiation rate of potatoes.The concentration of 6-BA in the highest callus growth of different potatoes was "Atlantic", "Desiree" and "Hutou" 1 mg/L, "Favorite" and "Sharpdi" 2 mg/L, "Zihuabai" 3 mg/L respectively;the GA₃ in the highest callus growth of different mediums was "Atlantic", "Favorite", "Sharpdi" and "Zihuabai" 5 mg/L, "Desiree" 0.02 and 0.15 mg/L, "Hutou" 0.02, 0.05 and 0.1 mg/L respectively.The highest callus growth in different potatoes was "Atlantic" 0.039 5 g, "Favorite" 0.037 5 g, "Sharpdi" 0.041 g, "Zihuabai" 0.039 6 g, "Desiree" 0.042 5 g and "Hutou" 0.039 5 g respectively.The 6-BA in the highest adventitious bud differentiation rate of potatoes was "Atlantic", "Favorite" and "Sharpdi" 2 mg/L, "Zihuabai" and "Desiree" 1 mg/L, "Hutou" 0.1 mg/L respectively.The GA₃ in the highest adventitious bud differentiation rate of potatoes was "Atlantic" 0 mg/L, "Favorite" 2 mg/L, "Sharpdi" 1 mg/L, "Zihuabai" 5 mg/L, "Desiree" and "Hutou" 0.1 mg/L respectively.The highest adventitious buds differentiation rates in different mediums were "Atlantic" 18.18%, "Favorite" 13.04%, "Sharpdi" 17.86%, "Zihuabai" 15.00%, "Desiree" 44.44% and "Hutou" 47.83% respectively.The optimal medium for rooting induction was 1/2 MS+0.1 mg/L NAA, and the rooting rates were "Atlantic" 95.2%, "Favorite" 83.3%, "Sharpdi" 92.4%, "Zihuabai" 94.8%, "Desiree" 96.7% and "Hutou" 95.6%.The survival rate of six potatoes was all above 90%.

Key words: potato, callus, adventitious bud, hormone combination, regeneration