SMF2和T39代谢产物对国槐根茎腐烂病致病镰刀菌的抑菌活性

doi:10.16036/j.issn.1000-2650.202305239

摘要/Abstract

摘要： 目的: 研究长枝木霉SMF2和哈茨木霉T39的不同培养液和培养时间的代谢产物和不同有机溶剂的萃取物对镰刀菌的抑菌活性,明确SMF2和T39抑菌活性物质的培养条件和提取方法。方法: 以引起国槐根茎腐烂病的尖孢镰刀菌（Fusarium oxysporum）、腐皮镰刀菌（F. solani）、多隔镰刀菌（F. decemcellulare）、木贼镰刀菌（F. equiseti）和F. keratoplasticum为靶标,采用菌丝生长速率法测定抑制率及毒力。结果: SMF2和T39的PD培养液对尖孢镰刀菌的抑制作用最强;以WBM为营养基质、培养5 d的SMF2培养液,以PD营养基质、培养7 d的T39培养液,抑菌效果最优;正丁醇为SMF2和T39的抑菌活性物质的最佳提取溶剂,分步提取法正丁醇提取物对尖孢镰刀菌的抑菌效果良好,EC₅₀仅为187.39和193.60 mg/L。结论: 本研究为深入研究木霉菌抑菌活性物质的提取方法、研制开发木霉生防制剂和先导化合物提供了理论依据。

关键词: 长枝木霉SMF2, 哈茨木霉T39, 镰刀菌, 培养液, 抑菌活性

Abstract: 【Objective】 This paper was conducted to study the antibacterial activities of metabolites of different culture media and incubation times of Trichoderma longibrachiatum SMF2 and T. harzianum T39 and extracts of different organic solvents on Fusarium, and to clarify the culture conditions and extraction methods of SMF2 and T39 bacteriostatic active substances. 【Method】 Fusarium oxysporum, F. Solani, F. decemcellulare, F. equiseti and F. Keratomoplasticum, which caused root rot disease on Sophora japonica Linn were used as the test target, and the inhibition rate and virulence were determined by mycelial growth rate method. 【Result】 The results showed that the PD culture medium of SMF2 and T39 had the strongest inhibitory effect against F. oxysporum. SMF2 culture medium with WBM as nutrient matrix cultured for 5 days,and T39 culture medium with PD nutrient matrix cultured for 7 days had the best bacteriostatic effect. N-butanol was the best extraction solvent of the bacteriostatic active substances of SMF2 and T39, and the step-by-step extraction N-butanol extract has a good antibacterial effect against F. oxysporum, and the EC₅₀ was only 187.39 and 193.60 mg/L. 【Conclusion】 This study provided a theoretical basis for further study of the extraction method of antibacterial active substances of Trichoderma and of the development of Trichoderma biocontrol preparations and lead compounds.

Key words: Trichoderma longibrachiatum SMF2, T. harzianum T39, Fusarium, culture medium, bacteriostatic activity

中图分类号:

S476.1

赵晓彤, 王桂清. SMF2和T39代谢产物对国槐根茎腐烂病致病镰刀菌的抑菌活性[J]. 四川农业大学学报, 2023, 41(4): 658-664.

ZHAO Xiaotong, WANG Guiqing. Bacteriostatic Activity of Metabolites SMF2 and T39 against Pathogenic Fusarium of Root Rot Disease on Sophora japonica[J]. Journal of Sichuan Agricultural University, 2023, 41(4): 658-664.

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