家兔肌肉组织中保守miRNA的鉴定及进化分析

doi:10.16036/j.issn.1000-2650.202302232

四川农业大学学报 ›› 2023, Vol. 41 ›› Issue (4): 713-718.doi: 10.16036/j.issn.1000-2650.202302232

家兔肌肉组织中保守miRNA的鉴定及进化分析

张翔宇^1#, 张翠霞^1#, 张凯², 袁定胜¹, 李钰莹¹, 邝良德¹, 李丛艳¹, 郑洁¹, 任永军¹, 郭志强¹, 唐丽¹, 季杨¹, 杨锐¹, 雷岷¹, 黄邓萍¹, 谢晓红¹, 杨超^1,*

1.四川省畜牧科学研究院/动物遗传育种四川省重点实验室,成都 610066;
2.四川省草原科学研究院,成都 611743

收稿日期:2023-02-20 出版日期:2023-08-28 发布日期:2023-08-31
作者简介:张翔宇,博士,副研究员,主要从事家兔遗传育种与饲养管理,E-mail：928742851@qq.com;张翠霞,硕士,副研究员,主要从事家兔遗传育种与饲养管理,E-mail：27616110@qq.com。#为并列第一作者。*责任作者：杨超,硕士,助理研究员,主要从事家兔育种与饲养管理,E-mail：327193420@qq.com。
基金资助:
科研院所科技成果转化项目（2021JDZH0019）; 四川省畜牧科学研究院基本科研业务专项资金（SASA202105）; 四川省科技计划项目应用基础研究（2021YJ0267）; 四川省自然科学基金（2023NSFSC0171）; 农业部国家现代农业产业技术体系（CARS-43-D-1）; 四川省畜牧科学研究院基本科研业务专项资金（SASA202305）

Identification and Evolutionary Analysis of the Conserved miRNAs in the Muscle Tissue of Rabbits

ZHANG Xiangyu^1#, ZHANG Cuixia^1#, ZHANG Kai², YUAN Dingsheng¹, LI Yuying¹, KUANG Liangde¹, LI Congyan¹, ZHENG Jie¹, REN Yongjun¹, GUO Zhiqiang¹, TANG Li¹, JI Yang¹, YANG Rui¹, LEI Min¹, HUANG Dengping¹, XIE Xiaohong¹, YANG Chao^1,*

1. Sichuan Animal Science Academy/Key Laboratory of Animal Genetics and Breeding in Sichuan Provinc,Chengdu 610066,China;
2. Sichuan Academy of Grassland Sciences,Chengdu 611743,China

Received:2023-02-20 Online:2023-08-28 Published:2023-08-31

摘要/Abstract

摘要： 目的: 旨在鉴定家兔肌肉组织中的保守miRNA,获取其表达谱并分析其进化特征,进而为研究保守性miRNA在家兔生长发育过程中的生物学功能提供理论依据。方法: 采用Solexa技术对家兔背最长肌组织中miRNA进行了测序和分析。结果: 通过序列比对鉴定出521个保守的miRNA序列,分属于238个不同的家族。其中miR-1、miR-133、miR-206和miR-499等肌肉特异性表达的miRNA在肌肉中均有表达,且除miR-499外的其它3个miRNA在所测定样本中均高表达。10个动物保守性miRNA家族在S86专门化父系肌肉基因组中出现了丢失。靶基因显著富集于13条信号通路,其中Focal adhesion和PI3K-Akt信号通路与肌肉发育密切相关。结论: 研究鉴定的影响肌肉发育的miRNA及其靶基因富集通路,将有助于家兔生长调控机制的研究,加快可用于标记辅助选择的分子标记的开发。

关键词: 家兔, Solexa 测序, 微小RNA, 生物信息学方法

Abstract: 【Objective】 Identification and evolutionary analysis of conserved miRNAs expression in rabbit muscle was studied, with the purposes of providing a theoretical basisfor the further studies of the biological functions of conserved miRNAs in rabbit growth and development activities. 【Method】 MiRNA libraries of table rabbit Longissimus muscle tissue were sequenced and analyzed via using Solexa technology. 【Result】 The results showed that 521 conserved miRNA sequences belonging to 238 different miRNA families were identified by sequence alignment among the obtained RNA pure sequences. miR-1, miR-133, miR-206 and miR-499 muscle-specific miRNAs were expressed in muscle. Except miR-499, the three other muscle-specific miRNAs were highly expressed in tables of rabbit muscle. 10 animal-conserved miRNA families were lost in the muscle genomes of S86 specialized paternal lines. The target genes were significantly enriched in 13 signaling pathways, of which the Focal adhesion and PI3K-Akt signaling pathways were closely associated with muscle development. 【Conclusion】 The identified and predicted miRNAs and their target gene enrichment pathways affecting muscle development will contribute to the study of growth regulatory mechanisms in table rabbit, which accelerates the development of molecular markers that can be used for marker assisted selection.

Key words: rabbits, Solexa sequencing, microRNA, bioinformatics method

中图分类号:

S829.1

张翔宇, 张翠霞, 张凯, 袁定胜, 李钰莹, 邝良德, 李丛艳, 郑洁, 任永军, 郭志强, 唐丽, 季杨, 杨锐, 雷岷, 黄邓萍, 谢晓红, 杨超. 家兔肌肉组织中保守miRNA的鉴定及进化分析[J]. 四川农业大学学报, 2023, 41(4): 713-718.

ZHANG Xiangyu, ZHANG Cuixia, ZHANG Kai, YUAN Dingsheng, LI Yuying, KUANG Liangde, LI Congyan, ZHENG Jie, REN Yongjun, GUO Zhiqiang, TANG Li, JI Yang, YANG Rui, LEI Min, HUANG Dengping, XIE Xiaohong, YANG Chao. Identification and Evolutionary Analysis of the Conserved miRNAs in the Muscle Tissue of Rabbits[J]. Journal of Sichuan Agricultural University, 2023, 41(4): 713-718.

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家兔肌肉组织中保守miRNA的鉴定及进化分析

Identification and Evolutionary Analysis of the Conserved miRNAs in the Muscle Tissue of Rabbits

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