硫化氢通过介导抗氧化系统提高谷子抗旱性

doi:10.16036/j.issn.1000-2650.202212210

摘要/Abstract

摘要： 目的: 探讨硫化氢是否通过介导抗氧化系统提高谷子抗旱性。方法: 选择晋谷21号谷子作为研究对象,采用土培的方法,研究4个不同处理（水、干旱、干旱+硫化氢、干旱+羟胺）分别对谷子幼苗的生长、形态、叶片受损程度、叶片丙二醛和过氧化氢含量、叶片抗氧化剂含量以及抗氧化酶活性的影响。结果: 干旱胁迫处理下,100 μmol/L的硫氢化钠显著促进了谷子幼苗的生长,并维持了其正常的形态;显著提高了幼苗的抗氧化剂含量以及抗氧化酶活性,致使叶片内丙二醛和过氧化氢的含量显著降低,从而减轻了叶片的受损害程度,增强了谷子的抗旱能力。结论: 信号分子硫化氢可以通过介导抗氧化系统提高谷子抗旱性,这为深入研究硫化氢的生理功能提供了参考依据,也为基于硫化氢信号的新型植物抗旱剂的研发提供了理论基础。

关键词: 干旱胁迫, 谷子, 硫化氢, 抗氧化系统

Abstract: 【Objective】 The objective of this study is to explore whether hydrogen sulfide can improve drought resistance of millet by mediating antioxidant system. 【Method】 We selected Jingu No.21 millet as the research material and used the method of soil culture to study the effects of four different treatments （water, drought, drought + hydrogen sulfide, drought + hydroxylamine） on the growth, morphology, and leaf damge of millet seedlings, contents of malondialdehyde and hydrogen peroxide in leaves, antioxidant content and antioxidant enzyme activity in leaves of millet seedlings. 【Result】 Under drought stress, 100 μmol/L sodium hydrosulfide significantly promoted the growth of millet seedlings and maintained their normal morphology; significantly increased the antioxidant content and antioxidant enzyme activity in seedlings, resulting in a significant decrease in the content of malondialdehyde and hydrogen peroxide in leaves, thereby reducing the degree of damage to leaves, and enhancing the drought resistance of millet. 【Conclusion】 The signaling molecule hydrogen sulfide can improve the drought resistance of millet by mediating the antioxidant system, which provides a reference for the in-depth study of the physiological function of hydrogen sulfide, and also provides a theoretical basis for the research and development of new plant drought resistance agents based on hydrogen sulfide signaling.

Key words: drought stress, millet, hydrogen sulfide, antioxidant system

中图分类号:

Q945.78

郝雪峰, 亢春霞, 景秀清, 张笑, 丁玉勤. 硫化氢通过介导抗氧化系统提高谷子抗旱性[J]. 四川农业大学学报, 2023, 41(4): 573-581.

HAO Xuefeng, KANG Chunxia, JING Xiuqing, ZHANG Xiao, DING Yuqin. Hydrogen Sulfide Improves Drought Resistance of Millet by Mediating Antioxidant System[J]. Journal of Sichuan Agricultural University, 2023, 41(4): 573-581.

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