葛根多糖对腹泻犊牛炎性因子含量、抗氧化能力、免疫功能、DAO活性及肠道菌群的影响

doi:10.16036/j.issn.1000-2650.202211226

四川农业大学学报 ›› 2023, Vol. 41 ›› Issue (4): 698-706.doi: 10.16036/j.issn.1000-2650.202211226

葛根多糖对腹泻犊牛炎性因子含量、抗氧化能力、免疫功能、DAO活性及肠道菌群的影响

吴海涛^1#, 莘余^2#, 吕尚揆², 何玉华³, 唐骏麒², 樊磊², 傅宏庆^1,*, 沈留红^2,*

1.江苏农牧科技职业学院,江苏泰州 225300;
2.四川农业大学动物医学院/动物疫病与人类健康四川省重点实验室,成都 611130;
3.成都市大邑县农村发展服务中心,成都 611330

收稿日期:2022-11-28 出版日期:2023-08-28 发布日期:2023-08-31
作者简介:吴海涛,硕士,讲师,主要从事临床兽医学教学及科研工作,E-mail：wuht1989@163.com;莘余,硕士研究生,E-mail： xy15031324924@163.com。#为并列第一作者。*责任作者：傅宏庆,副教授,主要从事动物疾病防控技术研究,E-mail：410804583@qq.com;沈留红,副教授,硕士生导师,主要从事临床兽医学教学和科研工作,E-mail：shenlh@sicau.edu.cn。
基金资助:
江苏农牧科技职业学院产业发展关键技术创新资助项目（NSF2021ZR01）; 四川农业大学本科科研兴趣培养计划（KY2023776）; 四川省科技厅科技计划项目（2019YJ0650）; 四川农业大学学科建设双支计划（03572174）

Effects of Pueraria Polysaccharides on Contents of Inflammatory Factors, Antioxidant Capacity, Immune Function, DAO Activity and Intestinal Microflora of Neonatal Diarrheic Calves

WU Haitao^1#, SHEN Yu^2#, LYU Shangkui², HE Yuhua³, TANG Junqi², FAN Lei², FU Hongqing^1,*, SHEN Liuhong^2,*

1. Jiangsu Agri-Animal Husbandry Vocational College, Taizhou 225300, Jiangsu China;
2. Key Laboratory of Animal Disease and Human Health of Sichuan Province/College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China;
3. Service Center of Dayi County Rural Development, Chengdu 611330, China

Received:2022-11-28 Online:2023-08-28 Published:2023-08-31

摘要/Abstract

摘要： 目的: 为研究葛根多糖对腹泻犊牛炎性、抗氧化、免疫、DAO活性及肠道菌群的影响。方法: 试验分别选取规模化牧场日龄、体重相近的健康（C组）和自然发病并表现典型腹泻症状（T组）的犊牛各12头,T组犊牛在牛奶中添加葛根多糖400 mg/（kg·头·d）,连续5 d,C组不添加,犊牛腹泻当天记为T₁组,给药第5天后记为T₂组,检测C组第1天、T₁组和T₂组血液炎性、免疫、抗氧化和肠道通透性指标以及肠道菌群多样性和丰富度。结果: ①葛根多糖可促进腹泻犊牛IL-1β含量极显著降低（P<0.01）,IL-2、TNF-α、DAO含量显著降低（P<0.05）,MDA含量显著降低趋势（0.05≤P<0.10）,TGF-β、GSH-Px、IgA含量极显著升高（P<0.01）。②葛根多糖可促进腹泻犊牛肠道菌群相对丰度在门水平上,拟杆菌门（Bacterioidetes）极显著升高（P<0.01）,放线菌门（Actinobacteria）显著升高（P<0.05）,变形菌门（Proteobacteria）显著降低（P<0.05）,而梭杆菌门（Fusobacteria）降低,厚壁菌门（Firmicutes）升高,但差异不显著（P>0.05）。在属水平,拟杆菌属（Bacteroides）极显著升高（P<0.01）、粪杆菌属（Faecalibacterium）显著升高（P<0.05）,狭义梭菌属1（Clostridium_sensu_stricto_1）显著降低（P<0.05）,而巨单胞菌属（Megamonas）升高,但差异不显著（P>0.05）。结论: 葛根多糖可减轻腹泻犊牛炎性反应,增强抗氧化能力,提高免疫功能,调节肠道通透性,促进肠道有益菌生长,抑制有害菌增殖,从而治疗犊牛腹泻。

关键词: 葛根多糖, 腹泻犊牛, 炎性, 抗氧化, 免疫, 肠道菌群

Abstract: 【Objective】 This study aimed to investigate the effects of Pueraria polysaccharides on contents of inflammatory factors, antioxidant capacity, immune function, DAO activity and intestinal microflora of diarrheic calves. 【Method】 A total of 12 healthy calves （group C） with similar body weight and age and naturally occurring 12 calves with typical diarrhea symptoms （group T） from the large-scale farm were selected in the experiment. Group T calves were fed with Pueraria polysaccharides 400 mg/（kg-head-day） in milk for 5 days, but not in group C. The calves were marked T₁ on the day of diarrhea and after the 5th day of administration as group T₂. The indexes of blood inflammation, immunity, antioxidation, intestinal permeability, as well as the diversity and abundance of intestinal flora in group C on the first day, T₁ and T₂ were detected. 【Result】 ① Pueraria polysaccharide can promote the levels of IL-1β significantly decreased （P<0.01）, IL-2, TNF- α and DAO significantly decreased （P<0.05）, MDA content showed a significant decreasing trend （0.05≤P<0.10）, and TGF-β, GSH-Px and IgA significantly increased （P<0.01） in diarrheic calves. ② After treated with Pueraria polysaccharides, the relative abundance of Bacterioidetes, Actinobacteria, Bacteroides and Faecalibacterium were increased （P<0.05）; while the relative abundance of Proteobacteria and Clostridium_sensu_stricto_1 was decreased. 【Conclusion】 Pueraria polysaccharide can reduce inflammatory reaction of diarrheic calves, enhance the antioxidation capacity, improve the immune function and regulate intestinal permeability. Moreover, it can also promote the growth of intestinal beneficial bacteria and inhibit the proliferation of harmful bacteria, so as to treat calf diarrhea.

Key words: Pueraria polysaccharides, neonatal diarrheic calves, inflammatory factors, antioxidant capacity, immune function, intestinal microflora

中图分类号:

S854.5

吴海涛, 莘余, 吕尚揆, 何玉华, 唐骏麒, 樊磊, 傅宏庆, 沈留红. 葛根多糖对腹泻犊牛炎性因子含量、抗氧化能力、免疫功能、DAO活性及肠道菌群的影响[J]. 四川农业大学学报, 2023, 41(4): 698-706.

WU Haitao, SHEN Yu, LYU Shangkui, HE Yuhua, TANG Junqi, FAN Lei, FU Hongqing, SHEN Liuhong. Effects of Pueraria Polysaccharides on Contents of Inflammatory Factors, Antioxidant Capacity, Immune Function, DAO Activity and Intestinal Microflora of Neonatal Diarrheic Calves[J]. Journal of Sichuan Agricultural University, 2023, 41(4): 698-706.

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葛根多糖对腹泻犊牛炎性因子含量、抗氧化能力、免疫功能、DAO活性及肠道菌群的影响

Effects of Pueraria Polysaccharides on Contents of Inflammatory Factors, Antioxidant Capacity, Immune Function, DAO Activity and Intestinal Microflora of Neonatal Diarrheic Calves

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