四川农业大学学报 ›› 2022, Vol. 40 ›› Issue (4): 543-549.doi: 10.16036/j.issn.1000-2650.202202098

• 动物科学 • 上一篇    下一篇

水温、流量、发育时期及孵化密度对澳洲淡水龙虾受精卵离体孵化率的影响

孙丽慧, 李倩, 陈建明, 姜建湖, 高令梅, 郭建林*   

  1. 浙江省淡水水产研究所/农业部淡水渔业健康养殖实验室/浙江省鱼类健康与营养重点实验室,浙江 湖州 313001
  • 收稿日期:2022-02-24 出版日期:2022-08-28 发布日期:2022-08-29
  • 通讯作者: *郭建林,高级工程师,主要从事水产动物营养与饲料研究,E-mail:wavegjl@aliyun.com。
  • 作者简介:孙丽慧,硕士,工程师,主要从事水产动物营养与饲料研究,E-mail: miduo1984422@126.com。
  • 基金资助:
    湖州市乡村振兴专项(2019ZD2028); 国家重点研发计划“蓝色粮仓科技创新”项目(2018YFD0901305)

Effects of Several Factors on inVitro Hatchability of Fertilized Eggs of Cherax quadricarinatus

SUN Lihui, LI Qian, CHEN Jianming, JIANG Jianhu, GAO Lingmei, GUO Jianlin*   

  1. Zhejiang Institute of Freshwater Fisheries, Key Laboratory of Healthy Freshwater Aquaculture, Ministry of Agriculture and Rural Affairs, Key Laboratory of Fish Health and Nutrition of Zhejiang Province,Huzhou 313001, Zhejiang, China
  • Received:2022-02-24 Online:2022-08-28 Published:2022-08-29

摘要: 【目的】 通过研究水温、流量、受精卵发育时期和孵化密度等因子的变化对澳洲淡水龙虾受精卵离体孵化率影响,以确定澳洲淡水龙虾受精卵离体孵化最适条件因子。【方法】 分别设计5个水温梯度、3个流量、3个发育时期及3个孵化密度进行澳洲淡水龙虾受精卵离体孵化试验。【结果】 随着水温升高,受精卵孵化时间呈下降趋势;受精卵离体孵化率随着水温的上升呈先上升后下降的趋势,在水温28 ℃,澳洲淡水龙虾受精卵离体孵化率达最高,显著高于其他试验组(P<0.05),复眼色素形成初期受精卵发育至初孵仔虾的平均有效积温为9 375 ℃·h。不同流量下澳洲淡水龙虾受精卵离体孵化率依次为5 L/min>2 L/min>8 L/min(P<0.05)。澳洲淡水龙虾受精卵发育至复眼色素形成初期和孵化准备期的孵化率显著高于前无节幼体期(P<0.05)。受精卵孵化密度为500粒/L和1 000粒/L时,离体孵化率显著高于1 500粒/L(P<0.05),但500粒/L和1 000粒/L时离体孵化率差异不显著(P>0.05)。【结论】 澳洲淡水龙虾受精卵离体孵化的适宜条件为:水温28 ℃,流量5 L/min,受精卵发育期为复眼色素形成初期,孵化密度为1 000粒/L(每个槽的尺寸为长×宽×高为15 cm×9.7 cm×12 cm,水深3 cm±0.5 cm,体积约0.4 L)。

关键词: 澳洲淡水龙虾, 水温, 流量, 发育时期, 孵化密度, 离体孵化率

Abstract: 【Objective】 This study was conducted to optimize the hatching conditions of Australian freshwater crayfish. 【Method】 Four single factor experiments were conducted to investigate the effects of water temperature, waterflow, development period and hatching density on in vitro hatching rate of fertilized eggs of Australian freshwater crayfish. 【Result】 The results showed that the incubation time decreased with the increase of water temperature, the highest in vitro hatching rate was observed at 28 ℃(P<0.05), the hatchability of fertilized eggs was significantly higher at 26 ℃ and 30 ℃ than that at 24 ℃ and 32 ℃(P<0.05), the accumulative temperature was 9 375 ℃·h. The in vitro hatching rate of fertilized eggs under different waterflow was 5 L/min>2 L/min>8 L/min (P<0.05); The hatching rate of fertilized eggs in the embryo with eye pigments and prepare-hatchling stage was significantly higher than that in the egg-nauplius stage (P<0.05); The hatching rate of fertilized eggs was significantly higher at density 500 eggs/L and 1 000 eggs/L than that at density 1 500 eggs/L(P<0.05), however, there was no significant difference in in vitro hatchability between 500 eggs/L and 1 000 eggs/L(P>0.05). 【Conclusion】 In summary, the fertilized eggs of Australian freshwater crayfish were stripped in the embryo with eye pigments, at the density 1 000 eggs/L, at water temperature 28 ℃ and waterflow 5 L/min could obtain a higher hatching rate.

Key words: Cherax quadricarinatus, water temperature, waterflow, developmental period, hatching density, in vitro hatching rate

中图分类号: 

  • S966.12