四川农业大学学报 ›› 2022, Vol. 40 ›› Issue (4): 529-535.doi: 10.16036/j.issn.1000-2650.202201070

• 动物科学 • 上一篇    下一篇

热应激对不同泌乳阶段奶牛应激、抗氧化和肝功能相关指标的影响

姚志兰1#, 程李杰2#, 沈留红2, 曹随忠2, 王绍熙3, 傅宏庆1,*   

  1. 1.江苏农牧科技职业学院,江苏 泰州 225300;
    2.四川农业大学动物医学院/动物疫病与人类健康四川省重点实验室/奶牛疾病研究中心,成都 611130;
    3.重庆市农业广播电视学校,重庆 401121
  • 收稿日期:2022-01-26 出版日期:2022-08-28 发布日期:2022-08-29
  • 通讯作者: *傅宏庆,副教授,主要从事动物疾病防控与研究,E-mail:hqfu@jsahvc.edu.cn。
  • 作者简介:姚志兰,硕士,讲师、兽医师,主要从事临床兽医学教学和研究工作,E-mail:1318575080@qq.com;程李杰,硕士研究生。#为并列第一作者。
  • 基金资助:
    四川省科技厅科技计划项目(2019YJ0650); 四川农业大学学科建设双支计划(03571303); 江苏农牧科技职业学院产业发展关键技术创新资助项目(NSF2021ZR01)

Effects of Heat Stress on Serum Indexes of Stress, Antioxidant and Liver Function of Cows at Different Lactation Stages

YAO Zhilan1#, CHENG Lijie2#, SHEN Liuhong2, CAO Suizhong2, WANG Shaoxi3, FU Hongqing1,*   

  1. 1. Jiangsu Agri-animal Husbandry Vocational College, Taizhou 225300, Jiangsu,China;
    2. The Key Laboratory of Animal Disease and Human Health of Sichuan Province/The Medical Research Center for Cow Disease/College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China;
    3. Chongqing Agricultural Broadcast and Television School, Chongqing 401121,China
  • Received:2022-01-26 Online:2022-08-28 Published:2022-08-29

摘要: 【目的】 探究热应激对不同泌乳阶段奶牛应激、抗氧化和肝功能相关指标的影响,以期为不同泌乳阶段奶牛更有效预防热应激提供理论支持。【方法】 选择四川省某规模化奶牛场热应激期泌乳牛856头和非热应激期泌乳牛896头,按泌乳天数分为新产(0~21 d)、泌乳前期(22~120 d)、泌乳中期(121~200 d)和泌乳后期(201~305 d),分别随机选择处于热应激中期和非热应激期体况、年龄、泌乳天数相近的各泌乳阶段健康经产(2~4胎次)泌乳奶牛10头,采集各试验牛尾静脉血,检测热应激蛋白70(Heat shock protein 70,HSP70)、皮质醇(Cortisol,Cor)、超氧化物歧化酶(Superoxide dismutase,SOD)、总抗氧化能力(Total antioxidant capacity,T-AOC)、丙二醛(Malondialdehyde,MDA)、游离脂肪酸(Non-esterified fatty acid,NEFA)、结合珠蛋白(Haptoglobin,HPT)和谷氨酸脱氢酶(Glutamate dehydrogenase,GDH)水平。【结果】 热应激各泌乳阶段奶牛应激指标HSP70、Cor水平和抗氧化指标SOD活性均极显著上升(P<0.01);新产、泌乳前期奶牛T-AOC水平极显著降低(P<0.01),泌乳中期奶牛T-AOC水平显著降低(P<0.05);新产奶牛MDA水平显著上升(P<0.05),泌乳前期、中期奶牛MDA水平极显著上升(P<0.01)。各泌乳阶段奶牛GDH活性极显著升高(P<0.01);新产奶牛、泌乳前期奶牛NEFA水平极显著提高(P<0.01),泌乳中期显著提高(P<0.05);新产、泌乳前期和中期奶牛HPT水平均极显著上升(P<0.01)。【结论】 不同泌乳阶段奶牛均受热应激影响而发生不同程度应激反应和氧化损伤,且肝功能均不同程度损伤,新产、泌乳前期和中期奶牛尤为严重。

关键词: 奶牛, 泌乳阶段, 热应激, 氧化损伤, 生化指标, 肝功能

Abstract: 【Objective】 To study serum stress, antioxidant and liver function related indexes of cows at different lactation stages during heat stress period. 【Method】 All cows, including 856 cows in the heat stress period and 896 cows in the non-heat stress period, were divided into four lactation stages according to days in milk: the late perinatal cows (0-21 d), the early lactation cows (22-120 d), the mid-lactation cows (121-200 d), and the late-lactation cows (201-305 d). 10 cows were randomly selected with similar body conditions, age and days in milk in the same lactation period (2-4 births) during mild heat stress and non-heat stress period from each lactation period, the test cattle were collected separately venous blood of bovine tail root and serum biochemical indexes include heat shock protein 70 (HSP70), cortisol (Cor), superoxide dismutase (SOD), total antioxidant capacity (T-AOC), malondialdehyde(MDA), non-esterified fatty acid (NEFA), haptoglobin(HP), glutamate dehydrogenase (GDH) were measured. 【Result】 Compared with cows at different stages in non-heat stress period, cows in mild heat stress period serum stress index HSP70, Cor concentration and antioxidant index SOD activity increased obviously significantly(P<0.01), T-AOC of late perinatal cows and early lactation cows was significantly obviously reduced (P<0.01), mid-lactation cows was significantly reduced (P<0.05). MDA of late perinatal cows increased significantly (P<0.05), MDA of early lactation cows and mid-lactation cows increased obviously significantly (P<0.01). GDH activity of each lactation cows was significantly obviously increased (P<0.01), NEFA concentration of late perinatal cows and early lactation cows was significantly extremely increased (P<0.01), significant improvement in middle lactation (P<0.05), HP concentration of late perinatal cows, early and middle lactation cows was significantly increased (P<0.01). 【Conclusion】 The cows at different lactation stages were affected by heat stress, all of them had different degrees of stress reaction and oxidative damage, and the liver function was damaged to different degrees. In particular, early lactation cows and mid-lactation cows were seriously affected by heat stress.

Key words: cows, lactation period, heat stress, oxidative damage, biochemical indexes, liver function

中图分类号: 

  • S815.4