杉木细根AMF侵染特征和锰、镉含量对短期氮添加的响应

doi:10.16036/j.issn.1000-2650.202201046

摘要/Abstract

摘要： 【目的】深入认识氮沉降对杉木（Cunninghamia lanceolata）细根丛枝菌根真菌（arbuscular mycorrhizal fungi, AMF）侵染特征和重金属富集效率的影响,可为杉木人工林的氮素管理提供科学依据。【方法】以11 a和18 a杉木人工林为对象,设置3个氮沉降梯度：CK、LN和HN,即0、5和10 g N/（m²·a）,通过施氮的方式模拟氮沉降,分析短期氮添加对AMF侵染特征和细根中锰、镉含量的影响。【结果】 ①氮添加对杉木AMF泡囊、菌丝和总侵染率以及土壤易提取球囊霉素含量影响显著（P<0.05）,在生长季（8月）,两种林龄以上指标均表现为LN条件下最高;在非生长季（11月）,11 a林内,各侵染特征仍为LN条件下最高,18 a林内,总AMF侵染率随氮添加量的增加而降低;②锰和镉两种元素在细根中的富集模式不同,各级根系中锰的含量与易提取球囊霉素存在显著的负相关关系,而镉含量更容易受到AMF侵染特征的影响。【结论】杉木细根与AMF的共生关系对短期氮沉降敏感,短期氮沉降同时影响着细根中重金属元素的富集与分配。

关键词: 氮沉降, 丛枝菌根真菌, 杉木人工林, 重金属

Abstract: 【Objective】 Deep insight into effects of N deposition on colonization of arbuscular mycorrhizal fungi （AMF） in fine roots of Cunninghamia lanceolata and heavy metals’ accumulation efficiency can provide scientific basis for N management of C. lanceolata plantations. 【Method】 In our study, two plots of C. lanceolata plantation （11 a and 18 a） were chosen as study objects, three nitrogen addition levels were set （CK, LN, HN, i.e., 0, 5, 10 g N/（m²·a））, nitrogen deposition has been simulated by continuous nitrogen addition every month. Colonization rate of AMF, Mn and Cd content in fine roots of C. lanceolata were analyzed after 4 and 7 months. 【Result】 ①short-term N addition have exerted significant effects on total colonization rate of AMF, colonization intensity of vesicles and hypha, and content of easily extracted glomalin in rhizosphere soil. In both plots, such parameters reach maximum under LN in summer. In winter, such infection characteristics were highest under LN in fine roots grown in 11 a C. lanceolata plot, while total colonization rate of AMF decreased with the increase of N amount in 18 a C. lanceolata plot; ②the accumulation pattern of Mn and Cd was different in fine roots of C. lanceolata and showed different responses to N deposition. Mn content in roots of C. lanceolata negatively correlated with content of easily extracted glomalin in rhizosphere soil, whereas Cd content in roots were affected significantly by AMF colonization. 【Conclusion】 The symbiotic relationship between AMF and fine roots of C. lanceolata is sensitive to the short-term N addition, which simultaneously affects accumulation and translocation of heavy metals in fine roots.

Key words: N deposition, arbuscular mycorrhizal fungi, Cunninghamia lanceolata plantation, heavy metals

中图分类号:

S718.51

陈良华, 刘选茹, 李西悦, 柳奇, 杨林凯, 舒锟, 林恬恬. 杉木细根AMF侵染特征和锰、镉含量对短期氮添加的响应[J]. 四川农业大学学报, 2022, 40(2): 233-242.

CHEN Lianghua, LIU Xuanru, LI Xiyue, LIU Qi, YANG Linkai, SHU Kun, LIN Tiantian. The Effects of Short-Term N Addition on AMF Infection Characteristics and Mn and Cd Content in Fine Roots of Cunninghamia lanceolata[J]. Journal of Sichuan Agricultural University, 2022, 40(2): 233-242.

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