四川农业大学学报 ›› 2022, Vol. 40 ›› Issue (4): 536-542.doi: 10.16036/j.issn.1000-2650.202111087

• 动物科学 • 上一篇    下一篇

姜黄素对犬骨髓间充质干细胞迁移能力的影响

李韦瑶, 邓嘉强, 赵芳芳, 朱雪瑞, 曹随忠, 沈留红, 余树民*   

  1. 四川农业大学动物医学院,成都 611130
  • 收稿日期:2021-11-08 出版日期:2022-08-28 发布日期:2022-08-29
  • 通讯作者: *余树民,教授,博士生导师,主要从事生殖内分泌与动物细胞工程研究,E-mail: yayushumin@sicau.edu.cn。
  • 作者简介:李韦瑶,硕士研究生。
  • 基金资助:
    四川农业大学学科建设双支计划(035/2121993256)

Effects of Curcumin on Migration of Canine Bone Marrow Mesenchymal Stem Cells

LI Weiyao, DENG Jiaqiang, ZHAO Fangfang, ZHU Xuerui, CAO Suizhong, SHEN Liuhong, YU Shumin   

  1. College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China
  • Received:2021-11-08 Online:2022-08-28 Published:2022-08-29

摘要: 【目的】 探讨姜黄活性成分姜黄素(curcumin,Cur)对犬骨髓间充质干细胞(canine bone marrow mesenchymal stem cells, cBMSCs)迁移功能的影响。【方法】 取体外传代培养条件下cBMSC作为试验材料,分别设置P3-cBMSCs为P3组,P6-cBMSCs+Cur(0、0.1、1和10 μmol/L)为姜黄素处理组。采用细胞划痕实验、Transwell细胞迁移实验、微丝绿色荧光探针(Actin-Tracker Green-488)染色、RT-qPCR、细胞坏死与凋亡检测和细胞相对端粒长度检测来评估姜黄素对cBMSCs迁移能力的影响。【结果】 与P3组相比,P6-cBMSCs划痕愈合率和Transwell小室外表面细胞迁移数量随着传代次数增加而降低(P<0.01);且细胞圆合度较高,应力纤维弯曲且稀少;迁移相关因子MMP-2MMP-9的基因表达水平显著下调(P<0.01)。经姜黄素处理后,P6-cBMSCs迁移能力提高,细胞应力纤维数量和排列均得到改善;迁移相关因子(MMP-2CXCL8)的基因表达水平均显著上调(P<0.01);细胞凋亡和坏死延缓,细胞活力得到维持;细胞相对端粒长度缩短迟滞。【结论】 Cur可以改善cBMSCs因体外扩增培养而受损的迁移能力,且以1 μmol/L的浓度作用最佳。

关键词: 姜黄素, 犬骨髓间充质干细胞, 细胞迁移

Abstract: 【Objective】 This study aims to explore the effects of curcumin (Cur) on migration of canine bone marrow mesenchymal stem cells(cBMSCs). 【Method】 cBMSCs cultured in vitro were chosen as the experimental materials and divided into P3 group (P3-cBMSCs) and Cur group [P6-cBMSCS + Cur (0, 0.1, 1 and 10 μmol/L)]. The migration ability of cBMSCs was tested by wound healing assay, Transwell assay, microfilament green fluorescent staining, RT-qPCR, cell necrosis, apoptosis and relative telomere length detection after treated with Cur. 【Result】 Compared with the P3 group, the wound healing rate and the number of cells migrated to the surface of the Transwell chamber decreased with the increase of passage times in P6-cBMSCs (P<0.01). Rounded cells, decreased stress fibers and of migration-related genes (MMP-2 and MMP-9) mRNA expression were found in P6-cBMSCs as compared with the P3 group. The migration ability of P6-cBMSCs was promoted after treated with Cur. In addition, enhanced cell spreading area and the degree of stretching, increased stress fibers, the upregulation of migration-related genes (MMP-2 and CXCL8) mRNA expression, maintained viability, delayed apoptosis and necrosis and shortened cell relative telomere length were observed after treated with Cur. 【Conclusion】 Cur can enhance the migration capacity of cBMSCs and 1 μmol/L was optimum.

Key words: curcumin, canine bone marrow mesenchymal stem cells, cell migration

中图分类号: 

  • R392.12