四川农业大学学报 ›› 2022, Vol. 40 ›› Issue (4): 519-528.doi: 10.16036/j.issn.1000-2650.202110086

• 动物科学 • 上一篇    下一篇

转录组分析探讨揭示羊肚菌多糖ME-X抗S180肿瘤的分子机制

鲁艳1, 黄瑶1, 叶姿妤1, 周丽倩1, 刘欣岚2, 丁祥2, 侯怡铃1,*   

  1. 1.西华师范大学生命科学学院/西南野生动植物资源保护教育部重点实验室 四川 南充 637009;
    2.西华师范大学环境科学与工程学院 四川 南充 637009
  • 收稿日期:2021-10-26 出版日期:2022-08-28 发布日期:2022-08-29
  • 通讯作者: *侯怡铃, 教授, 主要从事生物化学与分子生物学研究, E-mail: biostart8083@126.com。
  • 作者简介:鲁艳,硕士研究生。
  • 基金资助:
    辐照保藏四川省重点实验室开放基金项目 (FZBC2020009); 四川省科技厅应用基础研究项目(2018JY0087); 四川省科技厅重点研发项目(2018NZ0055)

Transcriptomes Analysis Revealed the Molecular Mechanism about the Anti-S180 Tumor Activity of the Polysaccharide ME-X from Morchella esculenta

LU Yan1, HUANG Yao1, YE Ziyu1, ZHOU Liqian1, LIU Xinlan2, DING Xiang2, HOU Yiling1,*   

  1. 1. Key Laboratory of Southwest China Wildlife Resources Conservation, Ministry of Education/College of Life Science, China West Normal University, Nanchong 637009, Sichuan, China;
    2. College of Environmental Science and Engineering, China West Normal University, Nanchong 637009, Sichuan, China
  • Received:2021-10-26 Online:2022-08-28 Published:2022-08-29

摘要: 【目的】 为探究羊肚菌多糖(ME-X)对S180肿瘤细胞抑制作用及其基因表达的影响,探索ME-X抑制小鼠S180肿瘤表型下潜在的作用分子及主要信号通路。【方法】 以S180肿瘤小鼠为模型,先进行ME-X体内抑制小鼠S180肿瘤试验,再结合RNA-Seq测序技术对体内试验组中小鼠的S180肿瘤细胞进行测序并对得到的数据进行生物信息学分析。【结果】 羊肚菌多糖(ME-X)和甘露聚糖肽(mannatide)一样能够极显著(P<0.01)抑制小鼠S180肿瘤的生长,其抑瘤率可达53.81%。差异表达基因(differential expressed genes,DEGs)分析结果显示羊肚菌多糖抑制S180肿瘤的关键基因主要富集在PI3K-AKT信号通路中,该通路中的成纤维细胞生长因子(Fgf10)、成纤维细胞生长因子受体(Fgfr2)、细胞因子受体(PrlrGhrI12rb)、磷酸烯醇式丙酮酸羧激酶(Pck1)等多个基因显著上调。【结论】 羊肚菌多糖(ME-X)可能通过PI3K-AKT信号通路调控S180肿瘤细胞代谢、迁移、细胞周期等进程达到抑制S180肿瘤的目的,为羊肚菌多糖抑制肿瘤分子机制研究提供可参考的理论依据。

关键词: 羊肚菌多糖, S180肿瘤, RNA-Seq, 差异表达基因, PI3K-AKT信号通路

Abstract: 【Objective】 This study aims to explore the antitumor activity of a polysaccharides from Morchella esculenta (named ME-X) and its impact on gene expression, observing the potential effect molecules and main signaling pathways under ME-X, which had a phenotype of suppressing the growth of S180 tumor in mice. 【Method】 In this study, the S180 tumor bearing mice were used as a model and antitumor experiments in mice were performed at first, then RNA-Seq technology was combined to sequence the S180 tumor cells from those mice in the experimental groups with bioinformatics analysis of the obtained data. 【Result】 ME-X, like Mannatide, which could significantly (P<0.01) inhibit the growth of S180 tumor in mice, and the rate of inhibition reached 53.81%. Differential expression genes (DEGs) analysis suggested that the key genes mostly enriched in the PI3K-AKT signaling pathway including many significantly up-regulated genes, such as fibroblast growth factor 10 (Fgf10), fibroblast growth factor receptor 2 (Fgfr2), cytokine receptors (Prlr, Ghr, I12rb) and phosphoenolpyruvate carboxykinase (Pck1). 【Conclusion】 It was suggested that ME-X could regulate the metabolism, migration and cell cycle of S180 tumor cells through PI3K-AKT signaling pathway to inhibit S180 tumor.

Key words: ME-X, S180 tumor, RNA-Seq, differential expressed genes (DEGs), PI3K-AKT signaling pathway

中图分类号: 

  • Q591