芹菜TCP基因家族全基因组鉴定、进化分析和表达研究

doi:10.16036/j.issn.1000-2650.202110039

摘要/Abstract

摘要： 【目的】鉴定芹菜基因组中TCP转录因子基因家族成员,为开展芹菜TCP基因功能研究及遗传改良提供理论依据。【方法】基于芹菜全基因组数据,对TCP家族成员进行了鉴定,系统分析了该家族成员的理化性质、基因结构、蛋白功能域、染色体分布、系统进化和转录表达丰度等,并采用qRT-PCR验证TCP参与了高温胁迫的应答。【结果】芹菜中共鉴定出29个TCP基因,将其划分为2个大的亚族Class Ⅰ与Class Ⅱ;TCP家族绝大多数基因位于染色体上且分布不均,只有2个基因位于重叠群上;所有成员均含有保守的结构域;进化分析发现伞形科作物的TCP基因亲缘关系较近;TCP家族成员的启动子区域显著富集与生长发育、激素响应和逆境应答相关的顺式元件;TCP家族大多数基因在芹菜叶片中高表达,且在白色、绿色和红色芹菜中的表达丰度都较高,且Class Ⅰ亚族还参与了高温胁迫的应答。【结论】研究结果有助于进一步了解芹菜TCP家族成员的系统进化和基因功能,为今后芹菜的基因工程改良提供了一定的理论基础。

关键词: 芹菜, TCP转录因子, 系统进化, 表达分析, 高温胁迫

Abstract: 【Objective】 The members of TCP transcription factor family in celery genome were screened and identified, which provided a theoretical basis for the functional research of TCP gene and genetic improvement of celery. 【Method】 In this study, the TCP family members were identified based on the whole-genome data of celery, and their physical and chemical properties, gene structure, chromosome distribution, phylogenetic evolution, protein functional domain, and transcript abundance were also analyzed. qRT-PCR was further performed to verify the expression under high temperature stress. 【Result】 The results showed that a total of 29 TCP genes were identified in celery, which were divided into two major classes： Class I and Class II. Most of the TCP genes are located on the chromosomes and are unevenly distributed, and only two genes are located on the contigs. All members contain conserved structural domains. Evolutionary analysis showed that TCP genes of Apiaceae crops displayed closer relationships. Promoter analysis showed that the promoter region of TCP family members was significantly enriched in cis-elements related to growth and development, hormone response and abiotic/biotic response. Expression pattern analysis showed that most genes of the TCP family are highly expressed in celery leaves, and the expression abundance in white, green and red celery is high, and the Class I subgroup may also be involved in the response to high temperature stress. 【Conclusion】 The research results are helpful in further understanding the systematic evolution and gene function of celery TCP family members, and also providing a certain theoretical basis for the genetic engineering improvement of celery.

Key words: celery, TCP transcription factor, systematic evolution, expression analysis, high temperature stress

中图分类号:

S637.3

周瑾, 李洁, 李晓燕, 肖丹怡, 李梦瑶. 芹菜TCP基因家族全基因组鉴定、进化分析和表达研究[J]. 四川农业大学学报, 2022, 40(2): 145-155.

ZHOU Jin, LI Jie, LI Xiaoyan, XIAO Danyi, LI Mengyao. Genome-Wide Identification, Evolution and Expression Analysis of TCP Gene Family in Celery (Apium graveolens L.)[J]. Journal of Sichuan Agricultural University, 2022, 40(2): 145-155.

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