柚AP2/ERF转录因子的鉴定及其在柚采后表达分析

doi:10.16036/j.issn.1000-2650.202109037

四川农业大学学报 ›› 2022, Vol. 40 ›› Issue (2): 179-190.doi: 10.16036/j.issn.1000-2650.202109037

• 特别策划：“蔬·果·茶”研究专栏 • 上一篇下一篇

柚AP2/ERF转录因子的鉴定及其在柚采后表达分析

彭玉娇^1,2,3, 宋芳菲^1,2,3, 李美欣^1,2,3, 邵元元^1,2,3, 崔学宇^1,2,3,*, 胡林^1,2,3, 谭梦超^2,3, 阮红燕^2,3, 区燕丽⁴

1.南宁师范大学广西地标作物大数据工程技术研究中心,南宁 530001;
2.南宁师范大学北部湾环境演变与资源利用教育部重点实验室,南宁 530001;
3.南宁师范大学广西地表过程与智能模拟重点实验室,南宁 530001;
4.容县土肥植保经作站,广西容县 537500

收稿日期:2021-09-07 出版日期:2022-04-30 发布日期:2022-05-05
通讯作者: ^*崔学宇,博士,助理研究员,主要从事果树生物技术研究,E-mail：yaoyuan200452@163.com。
作者简介:彭玉娇,硕士,助理研究员,主要从事果树生物技术研究,E-mail：1356346392@qq.com。
基金资助:
国家自然科学基金（32060294）; 广西自然科学基金（2020GXNSFAA297250）; 南宁师范大学大学生创新创业计划项目（202110603290,2022）; 广西高校中青年教师科研基础能力提升项目（2021KY0398）

Genomic Identification of Pomelo AP2/ERF Transcription Factors and Their Expression Pattern after Harvest

PENG Yujiao^1,2,3, SONG Fangfei^1,2,3, LI Meixin^1,2,3, SHAO Yuanyuan^1,2,3, CUI Xueyu^1,2,3,*, HU Lin^1,2,3, TAN Mengchao^1,2,3, RUAN Hongyan^2,3, OU Yanli⁴

1. Guangxi Geographical Indication Crops Research Center of Big Data Mining and Experimental Engineering Technology,Nanning Normal University,Nanning 530001,China;
2. Key Laboratory of Beibu Gulf Environment Change and Resources Utilization of Ministry of Education,Nanning Normal University,Nanning 530001,China;
3. Guangxi Key Laboratory of Earth Surface Processes and Intelligent Simulation,Nanning Normal University,Nanning 530001,China;
4. Soil and fertilizer station of the Agriculture Bureau of Rongxian County,Rongxian 537500,Guangxi,China

Received:2021-09-07 Online:2022-04-30 Published:2022-05-05

摘要/Abstract

摘要： 【目的】探究柚AP2/ERF转录因子在柚采后的表达模式,为研究柚采后品质变化可能的发生机制提供参考。【方法】通过测定沙田柚采后不同室温保存时间下主要糖、酸的变化,利用生物信息学的方法鉴定柚AP2/ERF转录因子家族成员,而后利用转录组测序和实时定量PCR的方法,对沙田柚采后AP2/ERF基因的表达动态进行分析。【结果】沙田柚采后总酸、柠檬酸和VC的含量均出现上升的趋势,苹果酸含量无显著变化,蔗糖含量呈下降趋势,葡萄糖和果糖的含量呈上升趋势;在柚的9条染色体上鉴定到了127个AP2/ERF转录因子,这些转录因子包括107个ERF基因、16个AP2基因、4个RAV基因和1个独立基因;共有63个基因在沙田柚采后过程中发生了差异表达,和刚采摘相比CgERF39表达量持续降低,CgERF1、CgERF63以及CgERF45表达量持续升高。【结论】证明了CgERF39、CgERF1、CgERF63和CgERF45是影响沙田柚采后品质的重要转录因子,为进一步明确沙田柚采后品质变化的机制提供依据。

关键词: 柚, AP2/ERF转录因子, 转录组测序

Abstract: 【Objective】 This paper aims to explore the expression pattern of AP2/ERF transcription factor in post-harvest pomelo,so as to provide reference for studying the possible mechanism of post-harvest quality changes in pomelo. 【Method】 In this study,the changes of major sugars and acids under different storage times at room temperature after harvest were measured,and the members of AP2/ERF transcription factor family of pomelo were identified by bioinformatics method,in addition,the expression dynamic of AP2/ERF genes after harvest by transcriptome date and real-time quantitative PCR were also analyzed. 【Result】 The results indicated that the contents of total acid,citric acid and VC all increased,while malic acid content had no significant change,sucrose content showed a downward trend,while glucose and fructose content showed an upward trend;127 AP2/ERF transcription factors were identified and distributed on 9 chromosomes of pomelo,including 107 ERF genes,16 AP2 genes,4 RAV genes and 1 independent gene;A total of 63 genes were differentially expressed in the post-harvest process of Shatian pomelo,and the expression levels of CgERF39 was continuously decreased,whereas the expressions of CgERF1,CgERF63 and CgERF45 were continuously increased compared with those in the harvest. 【Conclusion】 This study demonstrated that CgERF39,CgERF1,CgERF63 and CgERF45 were important transcription factors affecting post-harvest quality of Shatian pomelo,which provided basis for further clarifying the mechanism of post-harvest quality change of Shatian pomelo.

Key words: Citrus maxima, AP2/ERF transcription factors, transcriptome sequencing

中图分类号:

S666.3

彭玉娇, 宋芳菲, 李美欣, 邵元元, 崔学宇, 胡林, 谭梦超, 阮红燕, 区燕丽. 柚AP2/ERF转录因子的鉴定及其在柚采后表达分析[J]. 四川农业大学学报, 2022, 40(2): 179-190.

PENG Yujiao, SONG Fangfei, LI Meixin, SHAO Yuanyuan, CUI Xueyu, HU Lin, TAN Mengchao, RUAN Hongyan, OU Yanli. Genomic Identification of Pomelo AP2/ERF Transcription Factors and Their Expression Pattern after Harvest[J]. Journal of Sichuan Agricultural University, 2022, 40(2): 179-190.

参考文献

[1] 彭玉娇, 崔学宇, 覃礼蒙, 等. 不同地区沙田柚果园土壤细菌群落结构和多样性分析[J]. 四川农业大学学报, 2020, 38(6): 715-722, 741.
[2] 彭玉娇, 崔学宇, 谭梦超, 等. 不同立地条件沙田柚果园土壤微生物多样性分析[J]. 南方农业学报, 2020, 51(5): 1136-1144.
[3] 彭玉娇, 崔婷婷, 崔学宇, 等. 立地条件及空间冠层对沙田柚品质的影响[J]. 种子, 2019, 38(11): 129-132, 135.
[4] 彭玉娇, 崔学宇, 邵元元, 等. 不同树龄沙田柚果园土壤肥力、叶片养分和土壤细菌群落的特征[J]. 江苏农业学报, 2021, 37(2): 348-354.
[5] 崔学宇, 彭玉娇, 陈众峰, 等. 利用LC-MS技术解析沙田柚、琯溪蜜柚种子代谢物的差异[J]. 种子, 2021, 40(2): 1-6.
[6] 刁俊明, 钟创光. 沙田柚果实贮藏期水分变化与枯水的关系[J]. 核农学报, 1999(4): 32-37.
[7] 胡位荣, 刘顺枝, 江月玲, 等. 乙烯利处理对沙田柚采后果实品质及衰老的影响[J]. 果树学报, 2013, 30(3): 458-464,507.
[8] 李芳, 刘璐, 李俭, 等. 不同贮藏条件下5个柚品种的果实品质评价研究[J]. 中国果树, 2021(8): 43-47.
[9] 刘萍, 牛英, 邓光宙, 等. 室温和低温贮藏沙田柚果实的有机酸含量变化[J]. 南方农业学报, 2016, 47(9): 1542-1546.
[10] 马勇, 巴德仁贵, 哈斯阿古拉. 甜瓜ERF转录因子基因家族的全基因组鉴定与进化分析[C]. 中国遗传学会第九次全国会员代表大会暨学术研讨会论文集. 哈尔滨:中国遗传学会,2013: 91.
[11] ZENG J K, LI X, XU Q, et al.EjAP2-1,an AP2/ ERF gene, is a novel regulator of fruit lignification induced by chilling injury,via interaction with Ej MYB transcription factors[J]. Plant Biotechnology Journal, 2015, 13(9): 1325-1334.
[12] WANG C,XIN M,ZHOU X Y,et al.The novel ethylene-responsive factor CsERF025 affects the development of fruit bending in cucumber[J]. Plant Molecular Biology, 2017, 95(4/5): 519-531.
[13] RUMYANA K,FAYE M R,JACQUELINE B L,et al.Transcriptome and metabolite profiling show that apetala2a is a major regulator of tomato fruit ripening[J]. Plant Cell, 2011, 23: 923-941.
[14] FENG B H,HAN Y C,XIAO Y Y,et al.The banana fruit Dof transcription factor MaDof23 acts as a repressor and interacts with MaERF9 in regulating ripening-related genes[J]. Journal of Experimental Botany, 2016(8): 2263-2275.
[15] ZHANG A D,HU X,KUANG S,et al.Isolation,classification and transcription profiles of the ethylene response factors (ERFs) in ripening kiwifruit[J]. Scientia Horticulturae, 2016, 199: 209-215.
[16] 彭玉娇, 崔学宇, 崔婷婷, 等. 不同大小沙田柚品质差异及其分子机理探究[J]. 食品工业科技, 2020, 41(1): 50-55.
[17] 崔学宇, 李静, 彭玉娇, 等. 不同覆盖材料对‘沙田柚’果实品质及果园土壤细菌类型的影响[J]. 中国果树, 2021, 3:33-38, 50.
[18] 宋康华, 黎宇婷, 张鲁斌. AP2/ERF转录因子调控果实品质研究进展[J]. 热带作物学报, 2019, 40(5): 1032-1040.
[19] 曾宪录, 林文健, 吴广霞, 等. 沙田柚感官品质的定量描述分析研究[J]. 广东农业科学, 2019, 46(6): 23-29.
[20] 刘萍, 黄春霞, 邓光宙,等. 低温贮藏和薄膜贮藏对沙田柚果实品质的影响[J]. 北方园艺, 2015(20): 114-117.
[21] LI Y Z, CHENG Y J, YI H L, et al.Genetic diversity in mandarin landraces and wild mandarins from China based on nuclear and chloroplast simple sequence repeat markers[J]. Journal of Horticultural Science and Biotechnology,2006,81(3):371-378.
[22] GU C, GUO Z H, HAO P P, et al.Multiple regulatory roles of AP2/ERF transcription factor in angiosperm[J]. Botanical Studies, 2017, 58(1): 6-13.
[23] XIAO L, CHEN R B, WU Y, et al.Research progress on effect of AP2/ERF transcription factors in regulating secondary metabolite biosynthesis[J]. China Journal of Chinese Materia Medica, 2020, 45(22): 5412-5420.
[24] FENG K,HOU X L,XING G M, et al.Advances in AP2/ERF super-family transcription factors in plant[J]. Critical Reviews in Biotechnology, 2020, 40(6): 750-776.
[25] 宋康华, 黎宇婷, 张鲁斌. AP2/ERF转录因子调控果实品质研究进展[J]. 热带作物学报, 2019, 40(5): 208-216.
[26] LIU M, LIMAGOMES B, MILA I, et al.Comprehensive profiling of ethylene response factor expression identifies ripening-associated ERF genes and their link to key regulators of fruit ripening in tomato[J]. Plant Physiology, 2016, 170(3): 1732-1744.
[27] FAN Z Q, KUANG J F, FU C C, et al.The banana transcriptional repressor madear1 negatively regulates cell wall-modifying genes involved in fruit ripening[J]. Frontiers in Plant Science, 2016, 7: 1-13.
[28] JING Z, PENG R H, CHENG Z M, et al.Genome-wide analysis of the putative AP2/ERF family genes in Vitis vinifera[J]. Scientia Horticulturae, 2009, 123(1): 73-81.
[29] ZHAO J Y, LI W, GUO C H, et al.Genome-wide analysis of AP2/ERF transcription factors in carrot (Daucus carota L.) reveals evolution and expression profiles under abiotic stress[J]. Molecular Genetics & Genomics, 2015, 290(6): 2049.
[30] ZHUANG J, CAI B, PENG R H, et al.Genome-wide analysis of the AP2/ERF gene family in Populus trichocarpa[J]. Biochemical & Biophysical Research Communications, 2008, 317(3): 468-474.
[31] ITO T M,POLIDO P B,RAMPIM M C,et al.Genome-wide identification and phylogenetic analysis of the AP2/ERF gene superfamily in sweet orange (Citrus sinensis)[J]. Genetics & Molecular Research Gmr,2014, 13(3): 7839-7851.
[32] LI M Y,XU Z S,HUANG Y,et al.Genome-wide analysis of AP2/ERF transcription factors in carrot (Daucus carota L.) reveals evolution and expression profiles under abiotic stress[J]. Molecular Genetics & Genomics, 2015, 290(6): 2049-2061.
[33] SHU Y,YING L,ZHANG J,et al.Genome-wide analysis of the ap2/erf superfamily genes and their responses to abiotic stress in Medicago truncatula[J]. Frontiers in Plant Science,2016,6:1247.
[34] SHENG L,MA C,CHEN Y,et al.Genome-wide screening of ap2 transcription factors involving in fruit color and aroma regulation of cultivated strawberry[J]. Genes,2021,12(4):530.
[35] ZHANG Z,LI X.Genome-wide identification of AP2/ERF superfamily genes and their expression during fruit ripening of Chinese jujube[J]. Scientific Reports,2018,8(1):1-16.
[36] 宋丽, 于文杰, 刘阳, 等. 板栗种子淀粉积累过程中ABA响应的AP2/ERF转录因子家族分析[J/OL].分子植物育种:1-17[2021-06-20].http://kns.cnki.net/kcms/detail/46.1068.S.20210309.1409.008.html.

柚AP2/ERF转录因子的鉴定及其在柚采后表达分析

Genomic Identification of Pomelo AP2/ERF Transcription Factors and Their Expression Pattern after Harvest

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