四川农业大学学报 ›› 2022, Vol. 40 ›› Issue (1): 105-110.doi: 10.16036/j.issn.1000-2650.202109008

• 动科·植保 • 上一篇    下一篇

日粮精粗比对青海黑藏羊小肠营养物质转运载体基因表达水平的影响

周力1,2#, 张峰硕1#, 张春梅1, 杨葆春1, 桂林生1,*, 王志有1,*   

  1. 1.青海大学农牧学院,西宁 810016;
    2.宁夏大学农学院,银川 750021
  • 收稿日期:2021-09-08 出版日期:2022-02-28 发布日期:2022-02-28
  • 通讯作者: *桂林生,硕士生导师,副教授,主要从事动物遗传育种与繁殖研究,E-mail:guilinsheng1234@163.com;王志有,教授,主要从事动物营养与饲料研究,E-mail:275201004@qq.com。
  • 作者简介:周力,博士研究生;张峰硕,硕士研究生。#为并列第一作者。
  • 基金资助:
    青海省农业农村厅(藏羊均衡生产关键技术与示范推广,CK216327); 青海省林草局(青海湖-祁连山保护体系,K992138); 青海大学校地合作项目(黑藏羊高效繁育基地与产业化服务,2020GN118); 青海省羊产业平台(农业生产发展资金,CK2134607)

Effects of Dietary Forage to Concentrate Ratio on Gene Expression Level of Nutrient Transporter in Small Intestine of Qinghai Black Tibetan Sheep

ZHOU Li1,2#, ZHANG Fengshuo1#, ZHANG Chunmei1, YANG Baochun1, GUI Linsheng1,*, WANG Zhiyou1,*   

  1. 1. College of Agriculture and Animal Husbandry, Qinghai University, Xining 810016,China;
    2. College of Agriculture, Ningxia University, Yinchuan 750021,China
  • Received:2021-09-08 Online:2022-02-28 Published:2022-02-28

摘要: 【目的】研究不同精粗比日粮对育成黑藏羊小肠中小肽转运载体1(PEPT1)、钠-葡萄糖转运蛋白1(SGLT1)、葡萄糖转运蛋白1(GLUT1)以及酪氨酸蛋白激酶2(JAK2)基因mRNA表达丰度的影响。【方法】选择体重相近(10.45±0.96) kg且健康的2月龄断奶黑藏羊公羔60只,随机分3个处理组,每组20只,分别饲喂70%精料补充料+30%粗料(HC组)、50%精料补充料+50%粗料(MC组)和30%精料补充料+70%粗料(LC组)的试验日粮。饲喂120 d后,采集黑藏羊十二指肠、空肠和回肠的黏膜层组织测定其PEPT1、SGLT1、GLUT1JAK2基因的mRNA相对表达丰度。【结果】①十二指肠HC组PEPT1基因的mRNA表达水平显著高于LC组(P<0.05),HC组SGLT1GLUT1基因的mRNA表达水平极显著高于其他2组(P<0.01)。②空肠HC组SGLT1GLUT1基因的mRNA表达水平极显著高于MC组和LC组(P<0.01),同时与LC组相比,MC组GLUT1基因的mRNA表达水平显著提高(P<0.05)。③回肠HC组PEPT1基因的mRNA表达水平极显著高于其他2组(P<0.01)。【结论】日粮组成可显著影响黑藏羊的营养物质转运载体基因表达水平,可能会直接或间接影响小肠的代谢功能,其中日粮精粗比为70∶30时效果较好。

关键词: 藏羊, 日粮精粗比, 肠道, 营养物质转运载体, mRNA

Abstract: 【Objective】 This experiment was conducted to investigate the effects of different concentrate to forage ratio diets on mRNA levels of Peptide transporter 1 PEPT1, Sodium-dependent glucose transporters 1 SGLT1, Glucose transporter 1GLUT1) and Januskinase 2JAK2) in intestinal tissues of black Tibetan sheep. 【Method】 Sixty 2-month-old healthy black Tibetan male lambs with similar body weight (10.45±0.96)kg were randomly divided into 3 treatment groups with 20 lambs per treatment. Lambs were fed a total mixed diet with 70% concentrate+30% roughage (HC group), 50% concentrate+50% roughage (MC group) and 30% concentrate+50% roughage (LC group), respectively. After feeding for 120 days, the mucosal tissues of duodenum, jejunum and ileum of black Tibetan sheep were collected to determine the relative expression abundance of PEPT1, SGLT1, GLUT1 and JAK2 gene. 【Result】 The results showed as follows: ① In duodenum, the mRNA levels of PEPT1 in group HC was higher than that in group LC (P<0.05), and the mRNA expression of both SGLT1 and GLUT1 in group HC was significantly higher than that in the other two groups (P<0.01). ② In jejunum, the mRNA levels of PEPT1 in HC groups were significantly greater than those in MC and LC group (P<0.01), and the mRNA expression of JAK2 and SGLT1 in group MC was significantly lower than that in group HC (P<0.05). ③ In ileum, the mRNA levels of PEPT1 gene in group HC was significantly higher than that in the other two groups (P<0.01). 【Conclusion】 Dietary composition could significantly affect the expression of nutrient transporter gene in black Tibetan sheep, which may directly or indirectly affect the metabolic function of small intestine, and the effect is better when the concentrate to forage ratio is 70∶30.

Key words: Tibetan sheep, dietary forage to concentrate ratio, intestine, nutrient transporter, mRNA

中图分类号: 

  • S826.83