关键词: 生物有机肥, 玉米产量, 土壤有机质, 土壤酶活性

Abstract: 【Objective】 In order to investigate the effect of bio-fertilizer partially substituting chemical fertilizer on soil nutrient condition and enzyme activity, and improve the problems of soil fertilization reduction caused by excessive fertilizer application. 【Method】 A maize hybrid Xundan 20 was used in a field experiment with five treatments （CF, 100% chemical fertilizer N; BF5, 95% chemical fertilizer N + 5% bio-fertilizer N; BF10, 90% chemical fertilizer N + 10% bio-fertilizer N; BF20, 80% chemical fertilizer N+20% bio-fertilizer N; BF30, 70% chemical fertilizer N + 30% bio-fertilizer N）. The soil organic matter, total nitrogen, activities of urease, sucrose, alkaline phosphstase and catalase and maize yield were determined in different treatments. 【Result】 Bio-fertilizer combined with chemical fertilizer significantly increased soil organic matter and total nitrogen contents, of which BF30 shared the highest increases of 25.43% and 31.87%, respectively. Increases of soil available nutrient contents first increased and then decreased with the increasing of bio-fertilizer application rates. The highest available nitrogen, phosphorus and potassium were recorded in BF20 treatment. The increases of urase and catalase activities were 15.13%-24.72% and 76.83%-89.02% compared with CF treatment, respectively. Bio-fertilizer application markedly increased activities of soil sucrase and alkaline phospatase by 4.61%-28.93% and 6.96%-23.93%, respectively, of which the highest increases were observed in BF20 treatments. The maize yield was significantly increased by bio-fertilizer, and the highest increase was found in BF20, which reached 23.79%. 【Conclusion】 The soil fertility and maize grain yield were increased by bio-fertilizer, and it has a better effect that replacing 20% chemical fertilizer N with bio-fertilizer N in this region.

Key words: bio-fertilizer, maizeyield, soil organic matter, soilenzyme activity