石菖蒲抑菌物质的提取工艺及协同抑菌活性研究

doi:10.16036/j.issn.1000-2650.202104061

摘要/Abstract

摘要： 目的: 为了减少猕猴桃溃疡病植物源抗菌药剂的原材料的生产成本,使中药材的利用率最大化,并开发适合针对猕猴桃溃疡病菌的植物源复配抑菌剂。方法: 通过测量不同提取条件下的石菖蒲提取物对猕猴桃溃疡病菌的抑菌圈直径,得到石菖蒲的较佳提取工艺,并用响应面分析法筛选出石菖蒲、黄芩、丁香、广藿香、大蒜和牡丹6种中药材组成的最佳复配物。结果: 石菖蒲提取物的较佳工艺条件为：乙醇浓度81.171 %、提取温度54.730 ℃、料液比1∶25、提取温度50 ℃、超声功率700 W,此条件下的抑菌圈直径可达27.81 mm,复配抑菌剂的最佳配方工艺为：黄芩∶石菖蒲∶大蒜∶广藿香∶牡丹∶丁香=182.310∶306.104∶521.432∶453.106∶153.199∶362.565,在此条件下,1.0 g/mL的复配物实际试验测得的抑制圈直径可达33.860 mm。结论: 确定了石菖蒲提取物对猕猴桃溃疡病菌产生抑菌作用的较佳提取工艺,且同条件下,复配物有更强的抑菌作用。

关键词: 猕猴桃溃疡病, 石菖蒲提取物, 提取工艺, 复配物, 抑菌

Abstract: 【Objective】 In order to reduce the production cost of plant-derived antibacterial agents for kiwifruit ulcer disease,maximize the utilization rate of Chinese medicinal materials,and develop plant-derived compound antibacterial agents suitable for kiwifruit ulcer disease. 【Method】 By measuring the diameters of the inhibition zone of Acorus tatarinowii extract under different extraction conditions against Pseudomonas syringae,the better extraction technology of Acorus tatarinowii was obtained.The optimal compound of Acorus tatarinowii,Scutellaria baicalensis,Syzygium aromaticum,Pogostemon cablin, Allium sativum and paeonia suffruticosa was screened by response surface analysis （RSM）. 【Result】 The better technological conditions of Acorus tatarinowii extract were as follows：ethanol concentration of 81.171%,extraction temperature of 54.730 ℃,solid-liquid ratio of 1∶25,extraction temperature of 50 ℃,ultrasonic power of 700 W. Under these conditions,the diameter of the inhibition zone could reach 27.81 mm.The optimum formula of compound antibacterial agent was：Scutellaria baicalensis∶Scutellaria baicalensis∶Allium sativum∶Pogostemon cablin∶Paeonia suffruticosa∶Syzygium aromaticum=182.310∶306.104∶521.432∶453.106∶153.199∶362.565. Under these conditions,the diameter of inhibition ring measured by the 1.0 g/mL compound could reach 33.860 mm in the actual test. 【Conclusion】 The optimal extraction process of Acorus tatarinowii extract against kiwifruit ulcer disease was determined, and the compound had stronger antibacterial effect under the same conditions.

Key words: kiwi canker disease, Acorus tatarinowii extract, extraction process, distribution of content, bacteriostatic

中图分类号:

S567.9

贺富胤, 石浩, 李凤华, 王仁才. 石菖蒲抑菌物质的提取工艺及协同抑菌活性研究[J]. 四川农业大学学报, 2023, 41(4): 640-649.

HE Fuyin, SHI Hao, LI Fenghua, WANG Rencai. Study on Extraction Technology and Synergistic Antibacterial Activity of Antifungal Substances from Acorus tatarinowii[J]. Journal of Sichuan Agricultural University, 2023, 41(4): 640-649.

参考文献

[1] 龙云川, 贵州猕猴桃溃疡病病菌分离鉴定及其培养特性[J]. 江苏农业科学, 2017, 45(20): 114-117.
[2] 井赵斌, 杨宏勃, 杨玉旺, 等. 秦岭北麓猕猴桃主产区溃疡病病原菌的分离与鉴定[J]. 西北林学院学报, 2016, 31(5): 188-193.
[3] 李莎莎. 猕猴桃溃疡病相关细菌的鉴定及致病性研究[D]. 合肥: 安徽农业大学, 2013.
[4] 郑韵芳, 余阿妹, 许诗仪, 等. 石菖蒲挥发油体外抗菌活性及抗炎作用研究[J]. 海峡药学, 2015, 27(10): 260-263.
[5] 王联生, 潘菊华. 石菖蒲治疗抑郁症的研究进展[J]. 环球中医药, 2017(4): 509-512.
[6] 吴玉红. 石菖蒲药理作用的实验研究[J]. 当代教育实践与教学研究, 2018(8): 658.
[7] 李娟, 麻晓雪, 李顺祥, 等. 湖南产石菖蒲和水菖蒲乙醇提取物及其萃取组分抑菌活性的比较研究[J]. 中成药, 2014, 36(2): 393-396.
[8] 邓业成, 玉艳珍, 王萌萌, 等. 石菖蒲提取物及其初步分离物的抑菌活性研究[J]. 安徽农业科学, 2010, 38(15): 7836-7838, 7875.
[9] 张雁南, 宁志亮, 陈长武, 等. 丁香、甘草协同抑菌作用研究[J]. 食品科学, 2010, 31(21): 65-68.
[10] QIAO L, MURRAY B W, SHIMAZAKI M, et al.Synergistic inhibition of human α-1,3-fucosyltransferase V[J]. Journal of the American Chemical Society, 1996, 118(33): 7653-7662.
[11] 夏艳. 拮抗细菌与植物源提取物复配防治烟草青枯病研究[D]. 北京: 中国农业科学院, 2014.
[12] 曾令达, 彭惠莲, 宋冠华, 等. 5种植物乙醇提取物及其复配物对荔枝霜疫霉菌的抑菌活性[J]. 南方农业学报, 2016, 47(8): 1332-1337.
[13] 姬妍茹, 杨庆丽, 董艳, 等. 复配植物源杀菌剂对7种植物病原真菌离体抑制效果研究[J]. 黑龙江科学, 2016(1): 8-9.
[14] LI J,WU C, LI F,et al.Optimization of ultrasound-assisted water extraction of flavonoids from Psidium guajava leaves by response surface analysis[J]. Prep Biochem Biotechnol, 2019, 49(1): 21-29.
[15] 天津科技大学. 一种快速检测抑菌物质活性的微孔板生物检测方法: CN200810153179.9[P].2009-04-21.
[16] 刘铁秋, 孙雪丹, 卢发瑞, 等. 石菖蒲提取物的抑菌效果及其提取工艺的优化[J]. 中国生物制品学杂志, 2014, 27(1): 89-94.
[17] 石浩, 王仁才, 王琰, 等. 黄芩抑菌物的提取工艺及抑菌稳定性[J]. 湖南农业大学学报(自然科学版), 2019, 45(2): 199-204.
[18] 张梅, 康晓慧, 马林. 五种药用植物提取液抑菌作用研究[J]. 湖北农业科学, 2011, 50(4): 728-730.
[19] FU Y,ZU Y,CHEN L,et al.Antimicrobial activity of clove and rosemary essential oils alone and in combination[J]. Phytotherapy Research, 2007, 21(10): 989-994.
[20] THANISSERY R,KATHARIOU S,SMITH D.Rosemary oil,clove oil,and a mix of thyme-orange essential oils inhibit Salmonella and Campylobacter in vitro[J]. Journal of Applied Poultry Research, 2014, 23(2): 221-227.
[21] 侯媛媛. 大黄和黄芩抑菌活性物质追踪及其抑菌机理研究[D]. 上海: 上海海洋大学, 2015.
[22] 周琦, 张宝善, 韦露莎, 等. 魔芋飞粉生物碱的抑菌活性及抑菌机理研究[J]. 西北农林科技大学学报(自然科学版), 2019, 47(3): 121-128.
[23] 杨平, 黎芳靖, 罗嫚, 等. 在小檗碱作用下水稻细菌性条斑病菌生理及转录反应分析[J]. 植物保护学报, 2020, 47(5): 1005-1018.