四川农业大学学报 ›› 2022, Vol. 40 ›› Issue (2): 198-205.doi: 10.16036/j.issn.1000-2650.202104020

• 特别策划:“蔬·果·茶”研究专栏 • 上一篇    下一篇

枣和酸枣果实有机酸代谢差异研究

邓倩1,2, 王羊1, 邓群仙1,*, 张慧芬1, 夏惠3, 梁东3, 王全2   

  1. 1.四川农业大学园艺学院,成都 611130;
    2.四川省广安市邻水县经果技术推广所,四川 邻水 638500;
    3.四川农业大学果蔬研究所,成都 611130
  • 收稿日期:2021-04-22 出版日期:2022-04-30 发布日期:2022-05-05
  • 通讯作者: *邓群仙,博士,教授,研究方向为果树栽培理论与技术,E-mail:dqxlwj@sina.com。
  • 作者简介:邓倩,硕士研究生。
  • 基金资助:
    四川省学术和技术带头人培养资金(2015XDPY); 四川农业大学学科建设双支计划(035/1921993032); 德阳市重点科学技术研究项目(2018CKJ018)

Differences in the Organic Acid Metabolism of Ziziphus jujuba Mill. and Ziziphus acidojujuba Cheng et Liu Fruits

DENG Qian1,2, WANG Yang1, DENG Qunxian1,*, ZHANG Huifen1, XIA Hui3, LIANG Dong3, WANG Quan2   

  1. 1. College of Horticulture,Sichuan Agricultural University,Chengdu 611130,China;
    2. Linshui County Economic and Fruit Technology Promotion Institute,Linshui 638500,Sichuan,China;
    3. Institute of Pomology and Olericulture,Sichuan Agricultural University,Chengdu 611130,China
  • Received:2021-04-22 Online:2022-04-30 Published:2022-05-05

摘要: 【目的】 研究‘圆形小酸枣’和‘罗江调元枣’的有机酸代谢差异。【方法】 以不同酸含量的‘圆形小酸枣’和‘罗江调元枣’果实为试材,通过测定各发育时期有机酸组分、含量以及其代谢相关基因表达量,进而解析枣果实有机酸含量差异形成原因。【结果】 ‘圆形小酸枣’和‘罗江调元枣’含有苹果酸、柠檬酸、酒石酸和琥珀酸。两个品种的对比表明,柠檬酸含量不同导致‘圆形小酸枣’和‘罗江调元枣’有机酸含量出现较大差异。柠檬酸代谢相关基因表达表明,CS1、Aco2-1和NAD-IDH与柠檬酸代谢密切相关;与‘圆形小酸枣’相比,Aco2-1、NAD-IDH1-2和NAD-IDH5在‘罗江调元枣’果实发育中后期具有更高的表达量。苹果酸代谢相关基因表达表明,FUMPEPC4和MDH1与苹果酸代谢紧密相关;与‘圆形小酸枣’相比,PEPC4和FUM在‘罗江调元枣’果实发育中期具有更高的表达量,而MDH1在‘罗江调元枣’果实发育后期表达量更高。【结论】 调控柠檬酸降解基因Aco2-1和NAD-IDH的差异表达是导致‘圆形小酸枣’和‘罗江调元枣’有机酸含量出现差异的原因。

关键词: 枣, 酸枣, 有机酸代谢, 基因表达

Abstract: 【Objective】 The differences in organic acid metabolism between Ziziphus jujuba Mill. cv. ‘Luojiangtiaoyuanzao’ and Ziziphus acidojujuba Cheng et Liu cv. ‘Yuanxingxiaosuanzao’ fruits were studied. 【Method】 The different in organic acid contents and expression levels of metabolism-related genes in ‘Luojiangtiaoyuanzao’ and ‘Yuanxingxiaosuanzao’ fruits at different developmental stages were analyzed to determine the differences in organic acids. 【Result】 ‘Yuanxingxiaosuanzao’ and ‘Luojiangtiaoyuanzao’ contained malic, citric, tartaric, and succinic acids. A comparison showed that differences in the citric acid contents led to differences in the organic acid contents between the two cultivars. The expression levels of citric acid metabolism-related genes revealed that CS1, Aco2-1, and NAD-IDH were closely correlated with citric acid metabolism. Aco2-1, NAD-IDH1-2, and NAD-IDH5 were more highly expressed during the middle and late fruit developmental stages of ‘Luojiangtiaoyuanzao’ than ‘Yuanxingxiaosuanzao’. The expression levels of the malic acid metabolism-related genes FUM, PEPC4, and MDH1 were closely correlated with malic acid metabolism. However, FUM and PEPC4 were more highly expressed during the middle fruit developmental stage of ‘Luojiangtiaoyuanzao’ compared with ‘Yuanxingxiaosuanzao’, whereas MDH1 was more highly expressed during the late fruit developmental stage of ‘Luojiangtiaoyuanzao’. 【Conclusion】 The differential expression of Aco2-1 and NAD-IDH is responsible for the difference in organic acid content between ‘Yuanxingxiaosuanzao’ and ‘Luojiangtiaoyuanzao’ jujubes.

Key words: Chinese jujube, sour jujube, metabolism of organic acid, gene expression

中图分类号: 

  • S665.1