四川农业大学学报 ›› 2022, Vol. 40 ›› Issue (1): 36-41.doi: 10.16036/j.issn.1000-2650.202104014

• 作物·园艺 • 上一篇    下一篇

不同成熟度正红431种子抗逆指标及发芽率变化研究

贺振1, 曲比伍合2, 蔡林2, 柯永培1,2, 余学杰1,2, 丁天立1, 石海春1,2,*   

  1. 1.四川农业大学农学院,成都 611130;
    2.四川正红生物技术有限责任公司,四川 双流 610213
  • 收稿日期:2021-04-15 出版日期:2022-02-28 发布日期:2022-02-28
  • 通讯作者: *石海春,博士,教授,博导,主要从事玉米遗传育种和种子学的科研与教学工作,E-mail:haichun169@163.com。
  • 作者简介:贺振,硕士研究生。
  • 基金资助:
    四川省重点研发项目(2021YFYZ0017); 四川省科技成果转化示范项目(2021ZHCG0045)

Study on the Change of Stress Resistance Marker and Germination Rate of Zhenghong 431 Seeds with Different Maturity

HE Zhen1, QUBI Wuhe2, CAI Lin2, KE Yongpei1,2, YU Xuejie1,2, DING Tianli1, SHI Haichun1,2,*   

  1. 1. College of Agronomy,Sichuan Agricultural University,Chengdu 611130,China;
    2. Sichuan Zhenghong Bio Co.,Ltd., Shuangliu 610213,Sichuan,China
  • Received:2021-04-15 Online:2022-02-28 Published:2022-02-28

摘要: 【目的】明确在四川成都和西昌生产玉米品种正红431种子时适宜的收获期,为生产高质量种子提供技术支撑。【方法】通过2个基地不同成熟度正红431种子丙二醛(MDA)、过氧化物酶(POD)、超氧化物歧化酶(SOD)的活性和淀粉、蛋白质、脂肪的含量以及种子发芽率的变化来综合判断。【结果】成都基地正红431种子MDA含量和POD活性分别在授粉后35和40 d趋于稳定,SOD活性在授粉后45 d时最高;淀粉、蛋白质和脂肪含量分别在授粉后35、40和35 d趋于稳定;发芽率在授粉后30 d可达到90%以上,45 d达到100%。西昌基地正红431种子MDA含量在授粉后40 d时含量趋于稳定状态,POD和SOD活性分别在45~60 d和55~60 d处于高水平状态;淀粉、蛋白质和脂肪含量分别在授粉后40、45和45 d趋于稳定;种子发芽率在授粉后25 d的可达94%,60 d达到100%。【结论】综合抗逆指标、贮藏物质和发芽率来看,在四川成都和西昌生产正红431种子,其适宜的收获期分别为授粉后45和60 d。

关键词: 玉米, 种子生产, 酶活性, 贮藏物质, 发芽率, 收获期

Abstract: 【Objective】 The appropriate harvest period of the maize hybrids Zhenghong 431 in Chengdu and Xichang was determined,which will provide a technical support for the production of high-quality seeds. 【Method】 The appropriate harvest period was judged by comparing the activities of stress resistance marker of malondialdehyde (MDA),peroxidase (POD),superoxide dismutase (SOD),and the content of starch,protein,fat,as well as the seed germination rate. 【Result】 In Chengdu,the MDA and POD content of Zhenghong 431 tended to be stable at 35th and 40th days after pollination,respectively,while the SOD contents reached the peak at the 45th days after pollination. The starch,protein and fat content of Zhenghong 431 reached stable at 35th,40th and 35th days after pollination,respectively. The germination rate of Zhenghong 431 was over 90% at 30th days after pollination and reached 100% at 45th days after pollination. In Xichang,the MDA content of Zhenghong 431 tended to be stable at 40 days after pollination. The POD and SOD contents of Zhenghong 431 reached the peak at the 45-60th days,55-60th days after pollination,respectively. The starch,protein and fat content of Zhenghong 431 were stable at 40th,45th and 45th days after pollination,respectively. The germination rate of Zhenghong 431 reached 94% in 25th days and 100% in 60 days after pollination. 【Conclusion】 Combining the stress resistance marker,storage substance and germination rate,we conclude the suitable harvest periods for Zhenghong 431 seeds in Chengdu and Xichang are 45 days and 60 days after pollination,respectively.

Key words: maize, seed production, enzyme activity, storage material, germination rate, harvest periods

中图分类号: 

  • S513