黑藏羊不同部位肌纤维组织学特征的差异分析

doi:10.16036/j.issn.1000-2650.2021.06.010

摘要/Abstract

摘要： 【目的】分析不同部位肌肉的肌纤维特性,为充分挖掘青海黑藏羊（♂）的肉用潜质提供参考。【方法】利用ATP酶（ATPase）组织化学染色法和实时荧光定量聚合酶链式反应（real-time qPCR）测定黑藏羊背最长肌（longissimus dorsi, LD）、臂三头肌（biceps femoris muscle, BF）和股二头肌（Triceps femoris muscle, TF）3个部位肌肉的肌纤维组织学特征以及与其相关的肌球蛋白重链（Myosin heavy chains, MyHCs）基因的表达量。【结果】①背最长肌与臂三头肌ⅡA型、ⅡB型肌纤维直径显著低于股二头肌（P<0.05）;而股二头肌Ⅰ型、ⅡA型和ⅡB型肌纤维横截面积显著高于背最长肌（P<0.05）;Ⅰ型、ⅡA型和ⅡB型肌纤维密度均表现为背最长肌>臂三头肌>股二头肌（P>0.05）。②背最长肌ⅡA型肌纤维数量比例高于臂三头肌和股二头肌,其中背最长肌与股二头肌差异显著（P<0.05）;背最长肌Ⅰ型肌纤维面积比例高于臂三头肌（P>0.05）和股二头肌（P<0.05）。③背最长肌MyHCⅡA基因的表达量显著高于股二头肌（P<0.05）,较臂三头肌差异不显著（P>0.05）,各部位间MyHCⅠ、MyHCⅡB和MyHCⅡX基因的表达量差异均不显著（P>0.05）。【结论】揭示了黑藏羊不同部位肌肉的肌纤维特性差异,为今后黑藏羊的分档加工提供了理论参考。

关键词: 黑藏羊, 肌纤维特性, MyHCs, 肉品质, TMR

Abstract: 【Objective】 This study analyzed fiber characteristics in different parts of muscle to provide reference for full exploiting meat potential of Qinghai black Tibetan sheep(♂). 【Method】 Via ATPase histochemical staining and real-time quantitative polymerase chain reaction (qPCR), muscle fiber histological characteristics and MyHCs gene expression level from longissimus Dorsi (LD), biceps femoris (BF) and triceps femoris (TF) were detected in black Tibetan sheep. 【Result】 ①The diameter of type Ⅱ A and type Ⅱ B muscle fibers in LD and TB were significantly lower than that in BF (P<0.05). The cross-sectional area of type Ⅰ, Ⅱ A and ⅡB in BF were significantly higher than that of LD (P<0.05). The fiber density of type Ⅰ, type ⅡA and type ⅡB muscle was LD>TB>BF (P>0.05). ②The proportion of type ⅡA muscle fibers in longissimus dorsi muscle was higher than that in brachial triceps muscle and biceps femoris muscle, and the difference between longissimus dorsi muscle and biceps femoris muscle was significant (P<0.05). The proportion of type Ⅰ muscle fiber area in longissimus dorsi muscle was higher than that in brachial triceps (P>0.05) and biceps femoris (P<0.05). ③The gene expression level of MyHCⅡA in LD was significantly higher than that in BF (P<0.05), but had no significant difference compared with TB (P>0.05). The gene expression level of MyHCⅠ, MyHCⅡB and MyHCⅡX at different parts of LD had no significant difference (P>0.05). 【Conclusion】 This study revealed the distributional differences of fibromuscular characteristics in different parts, providing a theoretical reference for the grading processing of Black Tibetan sheep.

Key words: black Tibetan sheep, muscle fiber traits, MyHCs, meat quality, TMR

中图分类号:

S826.9+2

周力, 吴占月, 张峰硕, 桂林生, 侯生珍. 黑藏羊不同部位肌纤维组织学特征的差异分析[J]. 四川农业大学学报, 2021, 39(6): 778-783.

ZHOU Li, WU Zhanyue, ZHANG Fengshuo, GUI Linsheng, HOU Shengzhen. Analysis of Histological Characteristics of Muscle Fibers in Different Parts of Black Tibetan Sheep[J]. Journal of Sichuan Agricultural University, 2021, 39(6): 778-783.

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