永生化麝鼠香腺成纤维细胞的转录组学分析

doi:10.16036/j.issn.1000-2650.2021.06.009

四川农业大学学报 ›› 2021, Vol. 39 ›› Issue (6): 772-777.doi: 10.16036/j.issn.1000-2650.2021.06.009

永生化麝鼠香腺成纤维细胞的转录组学分析

竭航¹, 赵春容², 谌颖莲¹, 赵贵军¹, 张承露¹, 封孝兰¹, 曾德军^1,*

1.重庆市药物种植研究所/特色生物资源研究与利用川渝共建重点实验室,重庆 408435;
2.陆军军医大学,重庆 400038

收稿日期:2021-06-09 出版日期:2021-12-30 发布日期:2021-12-31
通讯作者: ^*曾德军,硕士,助理研究员,主要从事药用动物养殖研究,E-mail：113691516@qq.com。
作者简介:竭航,硕士,副研究员,主要从事药用动物学研究,E-mail：jiehangisgood@126.com。
基金资助:
重庆市基本科研业务费项目（cstc2020-jbky013）; 重庆市自然科学基金项目（cstc2019jcyj-msxmX0024）; 重庆市技术创新与应用发展项目（cstc2019jscx-dxwtBX0021）

Transcriptome Analysis of Immortalized Muskrat Scent Gland Fibroblasts

JIE Hang¹, ZHAO Chunrong², CHEN Yinglian¹, ZHAO Guijun¹, ZHANG Chenglu¹, FENG Xiaolan¹, ZENG Dejun^1,*

1. Chongqing Institute of Medicinal Plant Cultivation/Key Laboratory of Sichuan and Chongqing Co-construction for Characteristic Biological Resources Research and Utilization,Chongqing 408435,China;
2. Army Medicinal University,Chongqing 400038,China

Received:2021-06-09 Online:2021-12-30 Published:2021-12-31

摘要/Abstract

摘要： 【目的】探索永生化对麝鼠香腺成纤维细胞转录组的影响。【方法】在获得永生化的麝鼠香腺成纤维细胞后,采用二代测序的方法对其永生化前后转录组进行检测,并通过生物信息学方法进行差异分析。【结果】用Trinity软件对麝鼠永生化和原代香腺细胞测序数据进行基因组装,总共发现62 152 unigenes,富集分析结果显示,上述基因主要聚集在细胞代谢、细胞生物学调控、蛋白结合和酶催化等基因的细胞基础代谢活动。基于DESeq2包的基因表达差异分析结果表明,永生化和原代细胞表达差异的基因总共有8 690个,其中上调基因3 414个,下调基因5 276个,上调基因主要富集于DNA复制、同源重组、错配修复、细胞周期和RNA代谢通路,下调基因主要富集于氧化磷酸化、帕金森病和阿尔茨海默病疾病通路中。【结论】永生化过程对麝鼠原代细胞基因的影响较大,可为后续建立永生化香腺细胞体外泌香体系提供理论支撑。

关键词: 麝鼠, 香腺细胞, 永生化, 转录组

Abstract: 【Objective】 To explore the influence of immortalization on transcriptome of primary muskrat scent gland fibroblasts. 【Method】 Muskrat gland cells are the key to secreting muskrat musk. After obtaining the immortalized muskrat gland fibroblasts, in this study, transcriptome of muskrat immortalized and primary cells were detected through second-generation sequencing method, and the differences were analyzed by bioinformatics method. 【Result】 A total of 62 152 unigenes were found by gene assembling of the sequencing data of immortalized and primary cells using Trinity software. Cluster analysis showed that these genes were mainly enriched in cell metabolism, cell biological regulation, protein binding, enzyme catalysis and other basic metabolic activities. A total of 8 690 differentially expressed genes were detected according to DESeq2 analysis results, including 3 414 up-regulated and 5 276 down-regulated genes. And the up-regulated genesmainly occurred in DNA replication, raised homologous recombination, mismatch repair, cell cycle and RNA metabolism pathway, while down-regulated genes mainly enriched in the oxidative phosphorylation, Parkinson's and Alzheimer moss disease pathways in the disease. 【Conclusion】 Immortalization process had great influence on muskrat primary scent gland genes. This study provided theoretical support for the subsequent establishment of musk secretion system of immortalized scent glands cells in vitro.

Key words: Ondatra zibethicus, scent gland cell, immortality, transcriptome

中图分类号:

Q459

竭航, 赵春容, 谌颖莲, 赵贵军, 张承露, 封孝兰, 曾德军. 永生化麝鼠香腺成纤维细胞的转录组学分析[J]. 四川农业大学学报, 2021, 39(6): 772-777.

JIE Hang, ZHAO Chunrong, CHEN Yinglian, ZHAO Guijun, ZHANG Chenglu, FENG Xiaolan, ZENG Dejun. Transcriptome Analysis of Immortalized Muskrat Scent Gland Fibroblasts[J]. Journal of Sichuan Agricultural University, 2021, 39(6): 772-777.

参考文献 22

[1]	李宝唐. 麝鼠香化学成分的研究[J]. 中国药学杂志, 1994, 29(7): 396-397.
[2]	牛峻, 于艳秋, 杨英华, 等. 麝鼠泌香期有效成分大环酮类化合物的分析与研究[J]. 检验检疫科学, 1999, 9(3): 41-45.
[3]	赵伟刚, 陈玉山, 赵蒙, 等. 体外细胞分泌与自然分泌的麝鼠香化学成分比较研究[J]. 特产研究, 2009, 31(4): 50-53.
[4]	陈玉山, 金顺丹, 董万超, 等. 麝鼠香和天然麝香药理活性的比较研究[J]. 中药通报, 1988, 13(5): 46-47.
[5]	陈心智, 邱智东, 张永和, 等. 麝鼠香与麝香抗炎及镇痛作用的比较研究[J]. 吉林大学学报(医学版), 2005, 31(3): 414-416.
[6]	许文玉. 麝鼠香活血化瘀作用机制的研究[D]. 长春: 长春中医学院, 2005.
[7]	SHERESHKOV V L, SHUMILOVA T E, KUZMIN D A, et al.Effects of hypoxic factors on cardiac chronotropic reactions in the muskrat (Ondatra zibethicus) in free behavior[J]. Journal of Evolutionary Biochemistry and Physiology, 2006, 42(4): 461-468.
[8]	袁玲, 南一, 吴洋, 等. 麝鼠香对心肌缺血大鼠ET、 CGRP、VEGF的影响[J]. 宁夏医学杂志, 2010, 32(2): 117-119.
[9]	袁玲, 南一, 吴洋. 麝鼠香调控Bcl-2/Bax蛋白表达抑制缺血性大鼠心肌细胞凋亡[J]. 宁夏医科大学学报, 2010, 32(1): 1-3.
[10]	姜峰, 张学明, 韩小虎, 等. 麝鼠香囊腺形态学观察及腺上皮体外培养初报[J]. 中国农学通报, 2006, 22(11): 40-43.
[11]	GRABHERR M G, HAAS B J, YASSOUR M, et al.Full-length transcriptome assembly from RNA-Seq data without a reference genome[J]. Nature Biotechnology, 2011, 29(7): 644-652.
[12]	LOVE M I, HUBER W, ANDERS S.Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2[J]. Genome Biology, 2014, 15(12): 550.
[13]	YOUNG M D, WAKEFIELD M J, SMYTH G K, et al.Gene ontology analysis for RNA-seq: accounting for selection bias[J].Genome Biology, 2010, 11(2): R14.
[14]	MAO X, CAI T, OLYARCHUK J G, et al.Automated genome annotation and pathway identification using the KEGG Orthology (KO) as a controlled vocabulary[J]. Bioinformatics, 2005, 21: 3787-3793.
[15]	国家药典委员会. 中国药典: 一部[M]. 北京: 中国医药科技出版社, 2020: 412.
[16]	LU L, LIU S, LI Q, et al.Seasonal expression of androgen receptorin scented gland of muskrat (Ondatra zibethicus)[J]. General and Comparative Endocrinology, 2014, 204(8): 1-7.
[17]	ZHANG T, PENG D, QI L, et al.Musk gland seasonal development and musk secretion are regulated by the testis in muskrat (Ondatra zibethicus)[J]. Biological Research, 2017, 50(1): 10.
[18]	CUNHA G R, BOOUTIN E L, TURNER T, et al.Role of mesenchyme in the development of the urogenital tract[J]. Journal of Clean Technology Environmental Toxicology and Occupational Medicine, 1988, 7(2): 179-194.
[19]	李臣光, 安培培, 王大涛, 等. 麝鼠香腺发育及腺细胞培养分离的初步研究[J]. 兽类学报, 2020, 40(2): 175-182.
[20]	OBINATA M.Immortalized cell lines with differentiation potentials: their establishment and possible application[J]. Seikagaku, 2008, 80(1): 5-12.
[21]	李艳. 奶牛乳腺上皮细胞系的建立及生理功能鉴定[D]. 扬州: 扬州大学, 2011.
[22]	李嘉嘉. SFTSV感染靶细胞系的建立及转录组测序分析[D]. 合肥: 安徽医科大学, 2018.

永生化麝鼠香腺成纤维细胞的转录组学分析

Transcriptome Analysis of Immortalized Muskrat Scent Gland Fibroblasts

PDF (PC)

赞

可视化

摘要/Abstract

引用本文

使用本文

参考文献 22

相关文章 6

Metrics

本文评价

推荐阅读 10

[1]	蒲茜, 邱雪冰, 张镌钰, 汤燕燕, 王金慧, 李涛, 杨钊, 张海莉, 梁俊俊, 唐亚伟, 邓光兵, 龙海. 两种穗伸出差异显著的大麦最上节间全长转录组分析[J]. 四川农业大学学报, 2021, 39(4): 459-466.
[2]	武晶. 普通菜豆基因组研究进展[J]. 四川农业大学学报, 2021, 39(1): 4-10.
[3]	隋维恺, 高艳, 杨茜茜, 冷思竹, 严慧娟, 屈羽, 陈维刚, 杜小刚. PolyI:C刺激豚鹿(Axis porcinus)外周血淋巴细胞转录组分析[J]. 四川农业大学学报, 2021, 39(1): 114-120.
[4]	胡丹焱, 张正雪, 刘娟, 蓝增全, 吴田. 基于转录组测序的海巴戟果实中乙烯调控相关基因表达分析[J]. 四川农业大学学报, 2020, 38(05): 528-537.
[5]	陶军, 兰秀锦. 对具有一个共同亲本的杂种F₁育性差异的初步研究[J]. 四川农业大学学报, 2020, 38(01): 1-7.
[6]	李喜莲, 郭建林, 黄振远, 慎佩晶, 施伟达, 顾志敏. 红螯螯虾转录组高通量测序及分析[J]. 四川农业大学学报, 2019, 37(02): 241-246.