四川农业大学学报 ›› 2021, Vol. 39 ›› Issue (6): 734-741.doi: 10.16036/j.issn.1000-2650.2021.06.005

• 作物·园艺 • 上一篇    下一篇

枣和酸枣果实可溶性糖积累规律差异研究

邓倩1,2, 王羊1, 邓群仙1,*, 张慧芬1, 夏惠3, 林立金3, 吕小平2, 廖文飞2   

  1. 1.四川农业大学园艺学院,成都 611130;
    2.四川省广安市邻水县经果技术推广所,四川 邻水 638500;
    3.四川农业大学果蔬研究所,成都 611130
  • 收稿日期:2021-06-19 出版日期:2021-12-30 发布日期:2021-12-31
  • 通讯作者: *邓群仙,博士,教授,主要从事果树栽培理论与技术研究,E-mail: dqxlwj@sina.com。
  • 作者简介:邓倩,硕士研究生。
  • 基金资助:
    四川省学术和技术带头人培养资金(2015XDPY); 四川农业大学学科建设双支计划(035/1921993032); 德阳市重点科学技术研究项目(2018CKJ018)

Differences in Soluble Sugar Accumulation Pattern in Ziziphus jujuba Mill. and Ziziphus acidojujuba Cheng et Liu Fruits

DENG Qian1,2, WANG Yang1, DENG Qunxian1,*, ZHANG Huifen1, XIA Hui3, LIN Lijin3, LYU Xiaoping2, LIAO Wenfei2   

  1. 1. College of Horticulture,Sichuan Agricultural University,Chengdu 611130, China;
    2. Linshui County Economic and Fruit Technology Promotion Institute,Linshui 638500,Sichuan,China;
    3. Institute of Pomology and Olericulture,Sichuan Agricultural University,Chengdu 611130,China
  • Received:2021-06-19 Online:2021-12-30 Published:2021-12-31

摘要: 【目的】研究‘罗江调元枣’和‘圆形小酸枣’的可溶性糖积累规律。【方法】以味甜的‘罗江调元枣’和味酸的‘圆形小酸枣’果实为试材,通过测定不同发育时期可溶性糖组分含量及代谢相关基因表达量,进一步解析枣和酸枣果实可溶性糖积累规律差异的原因。【结果】‘圆形小酸枣’和‘罗江调元枣’含有蔗糖、果糖和葡萄糖;‘圆形小酸枣’为蔗糖和己糖共同积累型,‘罗江调元枣’为蔗糖积累型。两个品种的对比表明,果实发育后期蔗糖和己糖含量的变化导致‘圆形小酸枣’和‘罗江调元枣’可溶性糖积累规律不同。Zj SS1、Zj SS2、Zj SS3、Zj SPS1和Zj SPS2是‘圆形小酸枣’蔗糖合成的关键基因;Zj SS2、Zj SS3、Zj SPS1和Zj SPS2是‘罗江调元枣’蔗糖合成的关键基因;Zjv INV1、Zjc INV3和Zjn INV1是‘圆形小酸枣’果糖和葡萄糖合成的关键基因。【结论】‘罗江调元枣’和‘圆形小酸枣’果糖及葡萄糖的积累规律差异主要由Zjv INV1、Zjc INV3和Zjn INV1的差异表达所致,蔗糖的积累规律差异还有待进一步研究。

关键词: 枣, 酸枣, 糖代谢, 基因表达

Abstract: 【Objective】 Here, the differences in soluble sugar accumulations pattern between Ziziphus jujuba Mill. cv. ‘Luojiangtiaoyuanzao’ and Ziziphus acidojujuba Cheng et Liu cv. ‘Yuanxingxiaosuanzao’ fruits were studied. 【Method】 The fruits of ‘Luojiangtiaoyuanzao’ and ‘Yuanxingxiaosuanzao’ were used as test materials. The contents of soluble sugar components and the expression levels of metabolism-related genes at different developmental stages were analyzed to determine the different pattern of soluble sugar accumulation in jujube fruits. 【Result】 Both ‘Yuanxingxiaosuanzao’ and ‘Luojiangtiaoyuanzao’ contain sucrose, fructose and glucose. The fruits of ‘Yuanxingxiaosuanzao’ accumulated sucrose and hexose, whereas the fruits of ‘Luojiangtiaoyuanzao’ accumulated sucrose. A comparison showed that changes in the sucrose and hexose contents during late fruit development led to different soluble sugar accumulation pattern between the two cultivars. In the sucrose synthesis of ‘Yuanxingxiaosuanzao’, the sucrose metabolism-related genes Zj SS1, Zj SS2, Zj SS3, Zj SPS1 and Zj SPS2 played key roles, and sucrose metabolism-related genes Zj SS2, Zj SS3, Zj SPS1 and Zj SPS2 played key roles in ‘Luojiangtiaoyuanzao’. Zjv INV1, Zjc INV3 and Zjn INV1 played a key role in the synthesis of fructose and glucose in ‘Yuanxingxiaosuanzao’. 【Conclusion】 Thus, the differential expression of Zjv INV1, Zjc INV3 and Zjn INV1 is responsible for the different fructose and glucose accumulation pattern between ‘Yuanxingxiaosuanzao’ and ‘Luojiangtiaoyuanzao’ jujubes. The difference of sucrose accumulation pattern between ‘Yuanxingxiaosuanzao’ and ‘Luojiangtiaoyuanzao’ jujubes needs further study.

Key words: Chinese jujube, sour jujube, sugar metabolism, gene expression

中图分类号: 

  • S665.1