四川农业大学学报 ›› 2021, Vol. 39 ›› Issue (6): 721-728.doi: 10.16036/j.issn.1000-2650.2021.06.003

• 作物·园艺 • 上一篇    下一篇

基于GWAS的西瓜种子性状候选QTL鉴定

高美玲1,2, 胡创然1, 袁成志1, 郭宇1, 刘秀杰3, 刘继秀3, 高越3   

  1. 1.齐齐哈尔大学生命科学与农林学院,黑龙江 齐齐哈尔 161006;
    2.黑龙江省抗性基因工程与寒地生物多样性保护重点实验室,黑龙江 齐齐哈尔 161006;
    3.齐齐哈尔市园艺研究所,黑龙江 齐齐哈尔 161006
  • 收稿日期:2020-12-24 出版日期:2021-12-30 发布日期:2021-12-31
  • 作者简介:高美玲,博士,教授,主要从事瓜类作物分子育种及遗传改良研究,E-mail:gaomeiling0539@163.com。
  • 基金资助:
    国家自然科学基金项目(31772334、31972437); 黑龙江省省属高等学校基本科研业务费科研项目(135209106); 黑龙江省自然科学基金项目(LC2018015); 齐齐哈尔大学研究生创新科研项目(YJSCX2019048)

Identification of Candidate QTL for Watermelon Seed Traits Based on GWAS

GAO Meiling1,2, HU Chuangran1, YUAN Chengzhi1, GUO Yu1, LIU Xiujie3, LIU Jixiu3, GAO Yue3   

  1. 1. College of Life Science,Agriculture and Forestry,Qiqihar University,Qiqihar 161006,Heilongjiang,China;
    2. Heilongjiang Province Key Laboratory of Resistance Gene Engineering and Preservation of Biodiversity in Cold Areas,Qiqihar 161006,Heilongjiang,China;
    3. Qiqihar Horticultural Research Institute,Qiqihar 161006,Heilongjiang,China
  • Received:2020-12-24 Online:2021-12-30 Published:2021-12-31

摘要: 【目的】为探究控制西瓜种子大小的遗传机制。【方法】以144份纯合西瓜种质为试材,对多环境下种子大小相关性状进行多样性指数分析、相关性分析和全基因组关联分析。【结果】144份种质资源种子长度、种子宽度与种子百粒重两两之间均呈极显著正相关;多样性指数分别为4.69、4.78和4.80;全基因组关联分析检测到3个过阈值的QTL区域,分别位于4、7和10号染色体上,其中位于7号染色体上的种子大小性状相关QTL区域在多次环境中均被检测到。【结论】西瓜种子大小是复杂的数量性状,其QTL在多个染色体上均有分布。

关键词: 西瓜种子, 全基因组关联分析, 数量性状位点

Abstract: 【Objective】 To explore the genetic mechanism controlling the seed size of watermelon. 【Method】 Using 144 homozygous watermelon germplasms as test materials, the diversity index analysis,correlation analysis and genome-wide association analysis of seed size-related traits in multiple environments were carried out. 【Result】 There was a significant positive correlation between seed length,seed width and 100-seed weight of 144 germplasm resources; the diversity indexes were 4.69,4.78 and 4.80, respectively; the whole-genome association analysis detected 3 threshold values,the QTL regions of are located on chromosomes 4, 7 and 10,respectively. The QTL regions related to seed size traits on chromosome 7 have been detected in multiple environments. 【Conclusion】 Watermelon seed size is a complex quantitative trait,and its QTL is distributed on multiple chromosomes.

Key words: watermelon seed, genome-wide association analysis, quantitative trait locus

中图分类号: 

  • S651