水柏枝乙醇提取物不同极性部位体外抗氧化活性研究

doi:10.16036/j.issn.1000-2650.2021.04.012

摘要/Abstract

摘要： 【目的】探究水柏枝乙醇提取物不同极性部位的体外抗氧化活性。【方法】以水柏枝的70%乙醇提取物为样品,对醇提物进行萃取,得到水柏枝醇提物的不同极性部位并对其进行大类成分鉴别;用Folin-Ciocalteu方法和氯化铝比色法分别测定总多酚和总黄酮的含量;采用DPPH、ABTS和总还原能力3个指标来全面评估其体外抗氧化活性。【结果】初步植物化学筛选结果表明,水柏枝醇提物中不同程度地含有多酚、黄酮、香豆素、甾体和糖苷等类化合物,其中黄酮类在水柏枝的各个极性部位均有分布,酚类主要分布在乙酸乙酯和正丁醇部位,皂素主要分布于二氯部位,香豆素类主要集中在乙酸乙酯部位;萜类以及甾体主要分布在石油醚二氯部位。多酚和黄酮类含量测定结果表明,水柏枝不同极性部位当中,乙酸乙酯部位的多酚含量最高（94.43±1.5） mg/g,乙酸乙酯部位中总黄酮含量最高（627.21±1.2） mg/g。DPPH、ABTS和总还原能力3个指标综合得出水柏枝醇提物的乙酸乙酯部位的抗氧化能力最佳,正丁醇部位抗氧化能力次之。【结论】水柏枝乙醇提取物不同极性部位均有一定的抗氧化活性,其中乙酸乙酯部位活性最佳,且优于BHT,其良好的抗氧化能力可能与其中高含量的多酚和黄酮成分相关。

关键词: 水柏枝, 不同极性部位, 抗氧化, 定性鉴别, 定量测定

Abstract: 【Objective】 To explore the antioxidant activity of different polarity part of ethanol extract from Myricaria gerynanica (L.) Desv. in vitro. 【Method】 Taking the 70% ethanol extract of Myricaria gerynanica (L.) Desv. as a sample, the different polar parts were obtained by extraction with different solvent, which were identified the main chemicals; Folin-Ciocalteu method and aluminum chloride colorimetric method were used to determine the content of polyphenols and flavonoids. Three indicators, DPPH, ABTS, and total reducing capacity, were used to evaluate its anti-oxidant activity in vitro. 【Result】 Preliminary phyto-chemical screening results showed that the ethanol extract of Myricaria gerynanica (L.) Desv. contains polyphenols, flavonoids, coumarins, steroids, glycosides and other compounds to vary degrees, and the flavonoids are distributed in all polar parts of Myricaria gerynanica (L.) Desv., phenols are mainly distributed in ethyl acetate and n-butanol, saponins are mainly distributed in dichloride part, coumarins are mainly concentrated in ethyl acetate; terpenoids and steroids are mainly distributed in petroleum ether dichloride part. The results of determination of phenol and flavonoids content showed that the ethyl acetate part has the highest contents of total polyphenols (94.43±1.5) mg/g and total flavonoids (627.21±1.2) mg/g. The ethylacetate part exhibited the best antioxidant capacity in DPPH,ABTS, and total reduction capacity tests, then followed by n-butanol part. 【Conclusion】 All the different polar parts of the ethanol extract from Myricaria gerynanica (L.) Desv. exhibited certain antioxidant activity. Among them, the ethyl acetate part has the best antioxidant activity, better than B HT. Its good antioxidant capacity may own to the high content of polyphenols and flavonoids.

Key words: Myricaria gerynanica(L.) Desv., different polar parts, antioxidant, qualitative identification, quantitative determination

中图分类号:

S567.19

李芳龙, 阿呷尔布, 李家东, 梁晓霞. 水柏枝乙醇提取物不同极性部位体外抗氧化活性研究[J]. 四川农业大学学报, 2021, 39(4): 512-517.

LI Fanglong, AJIA Erbu, LI Jiadong, LIANG Xiaoxia. Study on the Antioxidant Activity in vitro of Different Polar Parts of Ethanol Extract of Myricaria gerynanica（L.） Desv.[J]. Journal of Sichuan Agricultural University, 2021, 39(4): 512-517.

参考文献 20

[1]	ALKADI H.A review on free radicals and antioxidants[J]. Infectious Disorders-Drug Targets, 2020, 20(1): 16-26.
[2]	汪启兵, 许凡萍, 魏超贤,等. 人体内自由基的研究进展[J].中华流行病学杂志, 2016, 37(8): 1175-1182.
[3]	GAŠPAROVIC A C. Free radical research in cancer[J]. Antioxi-dants, 2020, 9(2): 2-4.
[4]	KOLTOVER V K.Antioxidant biomedicine: from chemistry of free-radicals to reliability of biological systems[J]. Research in Medical & Engineering Sciences, 2018, 3(3): 565-565.
[5]	喇晓琴. 藏药翁布的化学成分及生物活性研究[D]. 西宁: 青海师范大学, 2011.
[6]	CHEN L, ZHANG X, HAO T, et al.Research progress on antioxidant activity of natural products[J]. European Journal of Bio Medical Research, 2016, 2(1): 36-40.
[7]	唐勋, 曾阳, 宋永朋, 等. 藏药翁布化学成分和药理学研究进展[J]. 青海师范大学学报 (自然科学版), 2014,12(2): 40-42.
[8]	BRAIN K R, TURNER T D.The practical evaluation of phytopharmaceuticals[M]. Bristol: Wright-Scientechnica, 1975.
[9]	MARIA R, SHIRLEY M, XAVIER C, et al.Preliminary phytochemical screening, total phenolic content and antibacterial activity of thirteen native species from Guayas province Ecuador[J]. Journal of King Saud University-Science, 2018, 30(4): 500-505.
[10]	AKHTAR N, MIRZA B.Phytochemical analysis and comprehensive evaluation of antimicrobial and antioxidant properties of 61 medicinal plant species[J]. Arabian Journal of Chemistry, 2018, 11(8):1223-1235.
[11]	KAIRUPAN C F, MANTIRI F R, RUMENDE R R H. Phytochemical screening and antioxidant activity of ethanol extract of leilem (Clerodendrum minahassae Teijsm. & Binn) as an antihyperlipidemic and antiatherosclerotic agent[J]. IOP Conference Series: Earth and Environmental Science, 2019, 217(1): 012016.
[12]	CHANG C C, YANG M H, WEN H M, et al.Estimation of total flavonoid content in propolis by two complementary colorimetric methods[J]. Journal of Food and Drug Analysis, 2002, 10(3): 178-182.
[13]	ALI T, NAQASH A, WADOO R, et al.Antimicrobial potential and determination of total phenolic and flavonoid content of aerial part extracts of Ajuga bracteosa Wall ex. Benth[J]. Pharmacognosy Communications, 2018, 8(3): 114-118.
[14]	余兰彬, 徐国良, 谭志伟, 等. DPPH法对5种抗衰老中药抗氧化活性的研究[J]. 江西中医药, 2016,47(8): 74-76.
[15]	ALESSANDRO T, ALESSANDRO C, MICHELE S, et al.The geek and the chemist: Antioxidant capacity measurements by DPPH assay in beverages using open source tools, consumer electronics and 3D printing[J]. Sensors and Actuators: B. Chemical, 2019,28(2): 584-591.
[16]	CHOU S T, CHAO W W, CHUNG Y C.Antioxidative activity and safety of 50% ethanolic red bean extract (Phaseolus radiatus L. var.Aurea)[J]. Journal of Food Science, 2003, 68(1): 21-25.
[17]	王蓓蓓, 段玉峰, 邵红军, 等. 红托竹荪多糖抗氧化活性的研究[J]. 天然产物研究与开发, 2012,24(8): 1122-1125.
[18]	GP, DWIECKI K, SIGER A, et al.Contribution of phenolic acids isolated from green and roasted boiled-type coffee brews to total coffee antioxidant capacity[J]. European Food Research and Tech-nology, 2016, 242(5): 641-653.
[19]	任婧. 黄刺玫果实醇提物的抗氧化及抗血栓作用实验研究[D]. 太原: 山西医科大学, 2017.
[20]	CHAHRA B, HAMAMA B, MAHFUZ E, et al.Antioxidant activity of polyphenolic leaf extract from Santolina chamaecyparissus L. (asteraceae) and the isolated luteolin-7-O-glucoside[J]. Journal of Pharmaceutical Research International, 2018, 22(3): 1-12.